|
Status |
Public on Mar 14, 2014 |
Title |
Adoptive transfer of OT-I derived T cells transduced with shRNA into B16 tumor bearing mice_4-sp2 |
Sample type |
RNA |
|
|
Source name |
CD8 T cells isolated from spleen
|
Organism |
Mus musculus |
Characteristics |
cell type: CD8 T cells transduction: shRNA targeting Ptpn2 tissue: spleen sample name: 4-sp2
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol, per manufacturers protocol
|
Label |
Biotin
|
Label protocol |
One cycle amplification by standard Affymetrix procedure
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol
|
Description |
CD8 T cells isolated from spleen; transduced with shRNA targeting: Ptpn2; Sample name: 4-sp2 Adoptive transfer of OT-I derived T cells transduced with shRNA into B16 tumor bearing mice.
|
Data processing |
JMP Genomics Background correction: RMA; Transformation: log2; Normalization: median scaling; Summary method: median
|
|
|
Submission date |
Dec 17, 2013 |
Last update date |
Mar 14, 2014 |
Contact name |
John R Walker |
E-mail(s) |
jwalker@gnf.org
|
Phone |
858-812-1636
|
Organization name |
Genomics Institute of the Novartis Research Foundation
|
Lab |
Genetics Core
|
Street address |
10675 John Jay Hopkins
|
City |
San Diego |
State/province |
CA |
ZIP/Postal code |
92121 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE53388 |
Immunotherapy Targets in Tumors |
|