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Sample GSM1287760 Query DataSets for GSM1287760
Status Public on Mar 31, 2014
Title GS-9_IFNb300_6h_rep1
Sample type RNA
Source name GS-9_IFNb300_6h_rep1
Organism Homo sapiens
Characteristics tissue: glioma
cell line: GS-9
Treatment protocol IFN-beta was dissolved in ddH2O. Cells were exposed to either IFN 300 IU/ml or ddH2O for either 6 h or 24 h before assembly of the cell pellets.
Growth protocol LNT-229 cells were grown in DMEM with 10% FCS and glutamine; GS-cells were grown in Neurobasal medium supplemented with growth factors and B27.
Extracted molecule total RNA
Extraction protocol RNA was extracted with the miRNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 217004); RNA qualitiy was checked with the 2100 BioAnalyzer (Agilent, Santa Clara, USA)
Label Biotin
Label protocol Sample preparation was done with 100 ng total-RNA using the GeneChip® 3´ IVT Express Kit (PN 901229) from Affymetrix (Santa Clara, USA) according to the instructions of the Affymetrix GeneChip® 3´ IVT Express Technical Manual (PN 702646). 10 µg of the fragmented cRNA were hybridized on the Gene Chip® Human Genome U133A 2.0 Arrays (Affymetrix, PN 900469)
Hybridization protocol Samples were hybridized for 16h at 45°C using Hybridization Kit materials (PN 900720, Affymetrix) according to the GeneChip® 3’ IVT Express Kit Protocol (PN 702646 Rev. 6, Affymetrix)
Scan protocol Affymetrix Gene Chip Scanner 3000 7G; AGCC
Data processing Quantile normalization / RMA summarization (GeneSpring 12.1, Agilent)
Submission date Dec 11, 2013
Last update date Mar 31, 2014
Contact name Karl Koehrer
Organization name University of Duesseldorf
Department BMFZ
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
Platform ID GPL571
Series (1)
GSE53213 Expression data from glioma cells exposed to interferon (IFN)-beta

Data table header descriptions
VALUE normalized RMA signal, log2

Data table
AFFX-BioB-5_at -0.25480938
AFFX-BioB-M_at -0.3414693
AFFX-BioB-3_at -0.27315998
AFFX-BioC-5_at -0.31119633
AFFX-BioC-3_at -0.32343864
AFFX-BioDn-5_at -0.36509037
AFFX-BioDn-3_at -0.14050007
AFFX-CreX-5_at -0.3131132
AFFX-CreX-3_at -0.1627407
AFFX-DapX-5_at -0.10299301
AFFX-DapX-M_at -0.09056091
AFFX-DapX-3_at -0.08965492
AFFX-LysX-5_at -0.0895772
AFFX-LysX-M_at -0.2243967
AFFX-LysX-3_at -0.12474251
AFFX-PheX-5_at -0.21682024
AFFX-PheX-M_at -0.12513304
AFFX-PheX-3_at -0.039783478
AFFX-ThrX-5_at 0.018371582
AFFX-ThrX-M_at -0.025758266

Total number of rows: 22277

Table truncated, full table size 487 Kbytes.

Supplementary file Size Download File type/resource
GSM1287760_3_GS-9_IFNb300_6h.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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