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Sample GSM1287757 Query DataSets for GSM1287757
Status Public on Mar 31, 2014
Title GS-2_Ctrl_24h_rep1
Sample type RNA
Source name GS-2_Ctrl_24h_rep1
Organism Homo sapiens
Characteristics tissue: glioma
cell line: GS-2
Treatment protocol IFN-beta was dissolved in ddH2O. Cells were exposed to either IFN 300 IU/ml or ddH2O for either 6 h or 24 h before assembly of the cell pellets.
Growth protocol LNT-229 cells were grown in DMEM with 10% FCS and glutamine; GS-cells were grown in Neurobasal medium supplemented with growth factors and B27.
Extracted molecule total RNA
Extraction protocol RNA was extracted with the miRNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 217004); RNA qualitiy was checked with the 2100 BioAnalyzer (Agilent, Santa Clara, USA)
Label Biotin
Label protocol Sample preparation was done with 100 ng total-RNA using the GeneChip® 3´ IVT Express Kit (PN 901229) from Affymetrix (Santa Clara, USA) according to the instructions of the Affymetrix GeneChip® 3´ IVT Express Technical Manual (PN 702646). 10 µg of the fragmented cRNA were hybridized on the Gene Chip® Human Genome U133A 2.0 Arrays (Affymetrix, PN 900469)
Hybridization protocol Samples were hybridized for 16h at 45°C using Hybridization Kit materials (PN 900720, Affymetrix) according to the GeneChip® 3’ IVT Express Kit Protocol (PN 702646 Rev. 6, Affymetrix)
Scan protocol Affymetrix Gene Chip Scanner 3000 7G; AGCC
Data processing Quantile normalization / RMA summarization (GeneSpring 12.1, Agilent)
Submission date Dec 11, 2013
Last update date Mar 31, 2014
Contact name Karl Koehrer
Organization name University of Duesseldorf
Department BMFZ
Street address Universitaetsstr. 1
City Duesseldorf
ZIP/Postal code 40225
Country Germany
Platform ID GPL571
Series (1)
GSE53213 Expression data from glioma cells exposed to interferon (IFN)-beta

Data table header descriptions
VALUE normalized RMA signal, log2

Data table
AFFX-BioB-5_at -0.25905085
AFFX-BioB-M_at -0.24461842
AFFX-BioB-3_at -0.25315762
AFFX-BioC-5_at -0.26395702
AFFX-BioC-3_at -0.25148487
AFFX-BioDn-5_at -0.16490746
AFFX-BioDn-3_at -0.16352844
AFFX-CreX-5_at -0.106594086
AFFX-CreX-3_at -0.09108448
AFFX-DapX-5_at -1.2494359
AFFX-DapX-M_at -1.1159954
AFFX-DapX-3_at -1.0461082
AFFX-LysX-5_at -0.94375086
AFFX-LysX-M_at -0.9434266
AFFX-LysX-3_at -1.1005311
AFFX-PheX-5_at -0.95286703
AFFX-PheX-M_at -1.2290006
AFFX-PheX-3_at -0.5425
AFFX-ThrX-5_at -1.185319
AFFX-ThrX-M_at -1.2003894

Total number of rows: 22277

Table truncated, full table size 489 Kbytes.

Supplementary file Size Download File type/resource
GSM1287757_6_GS-2_Ctrl_24h.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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