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Sample GSM128647 Query DataSets for GSM128647
Status Public on Oct 01, 2006
Title H157 siDJ-1 #2
Sample type RNA
 
Source name H157 siDJ-1 #2
Organism Homo sapiens
Characteristics NSCLC cell line H157. Transfected with siRNA oligomers targeting DJ-1 - #2
Treatment protocol H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule total RNA
Extraction protocol Promega SV Total RNA; DNAse I treated
Label biotin
Label protocol Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
 
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description Gene expression data from H157 cells transfected siRNA oligomers targeting DJ-1
Data processing Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
 
Submission date Aug 15, 2006
Last update date Sep 19, 2006
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
 
Platform ID GPL96
Series (1)
GSE5519 Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 318.9 P 0.002556
AFFX-BioB-M_at 934.7 P 0.000081
AFFX-BioB-3_at 425.7 P 0.00011
AFFX-BioC-5_at 1384.7 P 0.000195
AFFX-BioC-3_at 959.1 P 0.00007
AFFX-BioDn-5_at 991.8 P 0.000081
AFFX-BioDn-3_at 6112.4 P 0.000147
AFFX-CreX-5_at 12450.2 P 0.000044
AFFX-CreX-3_at 17166.6 P 0.000044
AFFX-DapX-5_at 5.8 A 0.574038
AFFX-DapX-M_at 35.7 A 0.354453
AFFX-DapX-3_at 5.7 A 0.804734
AFFX-LysX-5_at 22.8 A 0.195266
AFFX-LysX-M_at 8 A 0.852061
AFFX-LysX-3_at 24.5 A 0.327079
AFFX-PheX-5_at 3.3 A 0.910522
AFFX-PheX-M_at 6.7 A 0.852061
AFFX-PheX-3_at 71.1 A 0.368438
AFFX-ThrX-5_at 17 A 0.60308
AFFX-ThrX-M_at 19.3 A 0.5

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.




Supplementary data files not provided

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