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Sample GSM128644 Query DataSets for GSM128644
Status Public on Oct 01, 2006
Title H157 siCTL1
Sample type RNA
Source name H157 siCTL1
Organism Homo sapiens
Characteristics NSCLC cell line H157. Transfected with scrambeled control non-targetting oligomer siRNA - #1
Treatment protocol H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule total RNA
Extraction protocol Promega SV Total RNA; DNAse I treated
Label biotin
Label protocol Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description Gene expression data from H157 cells transfected with non-targetting scrambeled control siRNA oligomers
Data processing Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
Submission date Aug 15, 2006
Last update date Sep 19, 2006
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
Platform ID GPL96
Series (1)
GSE5519 Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 278.3 P 0.001248
AFFX-BioB-M_at 650.2 P 0.00007
AFFX-BioB-3_at 368.1 P 0.00034
AFFX-BioC-5_at 1174 P 0.000169
AFFX-BioC-3_at 818.7 P 0.000127
AFFX-BioDn-5_at 987.7 P 0.00006
AFFX-BioDn-3_at 5481 P 0.000169
AFFX-CreX-5_at 11836.3 P 0.000044
AFFX-CreX-3_at 15802.3 P 0.000044
AFFX-DapX-5_at 26.8 A 0.327079
AFFX-DapX-M_at 57.3 A 0.156732
AFFX-DapX-3_at 29.3 A 0.48511
AFFX-LysX-5_at 5.7 A 0.58862
AFFX-LysX-M_at 15.4 A 0.58862
AFFX-LysX-3_at 26.4 A 0.340661
AFFX-PheX-5_at 6.5 A 0.804734
AFFX-PheX-M_at 7.3 A 0.772364
AFFX-PheX-3_at 79.7 A 0.227636
AFFX-ThrX-5_at 17 A 0.51489
AFFX-ThrX-M_at 22.4 A 0.559354

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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