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Sample GSM128643 Query DataSets for GSM128643
Status Public on Oct 01, 2006
Title H157 siMOCK
Sample type RNA
Source name H157 siMOCK
Organism Homo sapiens
Characteristics NSCLC cell line H157. Transfected with Oligofectamine (Invitrogen) without RNA
Treatment protocol H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule total RNA
Extraction protocol Promega SV Total RNA; DNAse I treated
Label biotin
Label protocol Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description Gene expression data from H157 cells Mock transfected with transfection reagent alone.
Data processing Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
Submission date Aug 15, 2006
Last update date Sep 19, 2006
Contact name Casey M Clements
Organization name UNC Chapel Hill
Department Lineberger Cancer Center
Lab Jenny Ting's Lab
Street address CB#7295; LCCC 11-117
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7295
Country USA
Platform ID GPL96
Series (1)
GSE5519 Expression data from H157 non-small cell lung carcinoma cells with and without DJ-1 expression knocked-down

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 408.7 P 0.00762
AFFX-BioB-M_at 547.1 P 0.000127
AFFX-BioB-3_at 287.6 P 0.000857
AFFX-BioC-5_at 1029.7 P 0.000195
AFFX-BioC-3_at 658 P 0.000297
AFFX-BioDn-5_at 932.7 P 0.000081
AFFX-BioDn-3_at 5698 P 0.000195
AFFX-CreX-5_at 10297.1 P 0.000044
AFFX-CreX-3_at 16266.5 P 0.000044
AFFX-DapX-5_at 32.4 A 0.262827
AFFX-DapX-M_at 66.1 A 0.078002
AFFX-DapX-3_at 5.2 A 0.824672
AFFX-LysX-5_at 18.2 A 0.313723
AFFX-LysX-M_at 7.8 A 0.843268
AFFX-LysX-3_at 9.7 A 0.39692
AFFX-PheX-5_at 4.9 A 0.897835
AFFX-PheX-M_at 28.4 A 0.645547
AFFX-PheX-3_at 41.4 A 0.544587
AFFX-ThrX-5_at 15.6 A 0.699394
AFFX-ThrX-M_at 36.1 A 0.41138

Total number of rows: 22283

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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