NSCLC cell line H157. Transfected with Oligofectamine (Invitrogen) without RNA
Treatment protocol
H157 cells grown in RPMI w/ 10% FCS transfected 3 consecutive days annd GITC lysed 96 hours after first transfection. RNA isolated by column (Promega). Then cRNA made and labeled, hybridized to Affy U133A array
Extracted molecule
total RNA
Extraction protocol
Promega SV Total RNA; DNAse I treated
Label
biotin
Label protocol
Biotinylated cRNA were prepared using BioArray High Yield RNA Transcript Kit
Hybridization protocol
Following fragmentation, 10 microg of cRNA was hybridizd onto arrays for 16 hours at 45oC in the GeneChip Hybridization Oven 640
Scan protocol
Arrays were scanned with the Hewlett Packard GeneArray Scanner
Description
Gene expression data from H157 cells Mock transfected with transfection reagent alone.
Data processing
Affymetrix GeneChip Microarray Suite 5.0 software was used for washing, scanning, and basic analysis. Sample quality was assessed by examination of 3’ to 5’ intensity ratios of certain genes.
