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Sample GSM128421 Query DataSets for GSM128421
Status Public on Dec 31, 2006
Title proband 3 Tcells, before exercise
Sample type RNA
Source name human peripheral T-lymphocytes
Organism Homo sapiens
Characteristics human peripheral T-lymphocytes
Treatment protocol 8ml of whole blood were collected in BD Vacutainer CPT tubes. 400 µl of RosetteSep Tcell Enrichment cocktail were added immediately following the blood withdrawal. Tubes were inverted five times and incubated for 20 minutes at room temperature. Tubes were next centrifuged for 20 minutes at 1850g at room temperature. The cells were washed two times in icecold (4°C) PBS/ 2mM EDTA once in a volume of 50ml and the second time with a final volume of 5ml, spun down for 10 minutes at 500g at 4°C, the supernatant was removed by vacuum suction and the final pellet was lysed with Trizol.
Extracted molecule total RNA
Extraction protocol RNA was extracted from Trizol through addition of chloroform and phase separation using PLG tubes. Additionally RNA was cleaned up using the Rneasy Micro Kit with a Dnase step.
Label biotin
Label protocol biotinylated RNA was prepared from 3 µg total RNA according to the Affymetrix manual (2003)
Hybridization protocol 6.5 µg biotinylated RNA were hybridised for 16h at 45°C on geneChip human U133 A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics station 400.
Scan protocol GeneChips were scanned with the Affymetrix Scanner 3000.
Description proband 3 Tcells, before exercise
Data processing The data were first analysed with Affymetrix GCOS 1.2 using default analysis settings.
Submission date Aug 14, 2006
Last update date Oct 17, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE6053 Gene Expression analyses in PBMCs and T-lymphocytes following acute exhausting exercise

Data table header descriptions
ID_REF Affymetrix annotation number for the U 133 A 2.0 genome
VALUE GCOS calculated signal intensity
DETECTION P-VALUE p-value that indicates the significance of the detection call

Data table
AFFX-BioB-5_at 199.5 A 0.382599
AFFX-BioB-M_at 69.9 A 0.60308
AFFX-BioB-3_at 130 A 0.354453
AFFX-BioC-5_at 809.9 P 0.005565
AFFX-BioC-3_at 403 P 0.036569
AFFX-BioDn-5_at 719.7 P 0.001796
AFFX-BioDn-3_at 3508 P 0.00039
AFFX-CreX-5_at 7340.9 P 0.000044
AFFX-CreX-3_at 10160 P 0.000044
AFFX-DapX-5_at 447 P 0.000095
AFFX-DapX-M_at 798.1 P 0.000857
AFFX-DapX-3_at 819 P 0.000081
AFFX-LysX-5_at 71.8 P 0.028457
AFFX-LysX-M_at 88.6 A 0.156732
AFFX-LysX-3_at 138.6 P 0.000195
AFFX-PheX-5_at 52.3 A 0.095667
AFFX-PheX-M_at 63 M 0.050229
AFFX-PheX-3_at 183 A 0.0727
AFFX-ThrX-5_at 106.2 P 0.004998
AFFX-ThrX-M_at 154.3 P 0.004484

Total number of rows: 22277

Table truncated, full table size 593 Kbytes.

Supplementary data files not provided

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