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Sample GSM128416 Query DataSets for GSM128416
Status Public on Dec 31, 2006
Title proband 2 Tcells, before exercise
Sample type RNA
 
Source name human peripheral T-lymphocytes
Organism Homo sapiens
Characteristics human peripheral T-lymphocytes
Treatment protocol 8ml of whole blood were collected in BD Vacutainer CPT tubes. 400 µl of RosetteSep Tcell Enrichment cocktail were added immediately following the blood withdrawal. Tubes were inverted five times and incubated for 20 minutes at room temperature. Tubes were next centrifuged for 20 minutes at 1850g at room temperature. The cells were washed two times in icecold (4°C) PBS/ 2mM EDTA once in a volume of 50ml and the second time with a final volume of 5ml, spun down for 10 minutes at 500g at 4°C, the supernatant was removed by vacuum suction and the final pellet was lysed with Trizol.
Extracted molecule total RNA
Extraction protocol RNA was extracted from Trizol through addition of chloroform and phase separation using PLG tubes. Additionally RNA was cleaned up using the Rneasy Micro Kit with a Dnase step.
Label biotin
Label protocol biotinylated RNA was prepared from 3 µg total RNA according to the Affymetrix manual (2003)
 
Hybridization protocol 6.5 µg biotinylated RNA were hybridised for 16h at 45°C on geneChip human U133 A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics station 400.
Scan protocol GeneChips were scanned with the Affymetrix Scanner 3000.
Description proband 2 Tcells, before exercise
Data processing The data were first analysed with Affymetrix GCOS 1.2 using default analysis settings.
 
Submission date Aug 14, 2006
Last update date Oct 17, 2006
Contact name Sandy Mosig
E-mail(s) sandy.mosig@mti.uni-jena.de, sandy.mosig@googlemail.com
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
 
Platform ID GPL571
Series (1)
GSE6053 Gene Expression analyses in PBMCs and T-lymphocytes following acute exhausting exercise

Data table header descriptions
ID_REF Affymetrix annotation number for the U 133 A 2.0 genome
VALUE GCOS calculated signal intensity
ABS_CALL
DETECTION P-VALUE p-value that indicates the significance of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 181.6 P 0.002023
AFFX-BioB-M_at 234.8 P 0.000258
AFFX-BioB-3_at 78.4 A 0.116113
AFFX-BioC-5_at 593.2 P 0.000095
AFFX-BioC-3_at 702 P 0.000044
AFFX-BioDn-5_at 1312.3 P 0.000044
AFFX-BioDn-3_at 4452.5 P 0.000147
AFFX-CreX-5_at 10046.8 P 0.000052
AFFX-CreX-3_at 11542.7 P 0.000044
AFFX-DapX-5_at 664.8 P 0.000052
AFFX-DapX-M_at 1168.6 P 0.000754
AFFX-DapX-3_at 1202.3 P 0.00007
AFFX-LysX-5_at 90.9 P 0.002556
AFFX-LysX-M_at 126.7 P 0.033677
AFFX-LysX-3_at 218.8 P 0.000127
AFFX-PheX-5_at 134.1 P 0.00007
AFFX-PheX-M_at 161.7 P 0.001102
AFFX-PheX-3_at 193.3 P 0.005565
AFFX-ThrX-5_at 159.9 P 0.00039
AFFX-ThrX-M_at 218.1 P 0.000127

Total number of rows: 22277

Table truncated, full table size 591 Kbytes.




Supplementary data files not provided

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