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Sample GSM128413 Query DataSets for GSM128413
Status Public on Dec 31, 2006
Title proband 1 PBMC, before exercise
Sample type RNA
Source name human PBMC
Organism Homo sapiens
Characteristics human PBMC
Treatment protocol 8ml of whole blood were collected in BD Vacutainer CPT tubes. Tubes were next centrifuged for 20 minutes at 1850g at room temperature. The cells were washed two times in icecold (4°C) PBS/ 2mM EDTA once in a volume of 50ml and the second time with a final volume of 5ml, spun down for 10 minutes at 500g at 4°C, the supernatant was removed by vacuum suction and the final pellet was lysed with Trizol.
Extracted molecule total RNA
Extraction protocol RNA was extracted from Trizol through addition of chloroform and phase separation using PLG tubes. Additionally RNA was cleaned up using the Rneasy Micro Kit with a Dnase step.
Label biotin
Label protocol biotinylated RNA was prepared from 3 µg total RNA according to the Affymetrix manual (2003)
Hybridization protocol 6.5 µg biotinylated RNA were hybridised for 16h at 45°C on geneChip human U133 A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics station 400.
Scan protocol GeneChips were scanned with the Affymetrix Scanner 3000.
Description proband 1 PBMC, before exercise
Data processing The data were first analysed with Affymetrix GCOS 1.2 using default analysis settings.
Submission date Aug 14, 2006
Last update date Oct 17, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE6053 Gene Expression analyses in PBMCs and T-lymphocytes following acute exhausting exercise

Data table header descriptions
ID_REF Affymetrix annotation number for the U 133 A 2.0 genome
VALUE GCOS calculated signal intensity
DETECTION P-VALUE p-value that indicates the significance of the detection call

Data table
AFFX-BioB-5_at 220 P 0.001102
AFFX-BioB-M_at 228.4 P 0.000662
AFFX-BioB-3_at 123.6 P 0.009337
AFFX-BioC-5_at 673.2 P 0.00011
AFFX-BioC-3_at 790.9 P 0.000044
AFFX-BioDn-5_at 1434.3 P 0.000052
AFFX-BioDn-3_at 4920.3 P 0.000127
AFFX-CreX-5_at 11229.1 P 0.000052
AFFX-CreX-3_at 12518.1 P 0.000044
AFFX-DapX-5_at 555.6 P 0.00006
AFFX-DapX-M_at 899.4 P 0.001102
AFFX-DapX-3_at 897 P 0.000081
AFFX-LysX-5_at 55.4 P 0.006871
AFFX-LysX-M_at 108.1 A 0.0727
AFFX-LysX-3_at 115.7 P 0.000195
AFFX-PheX-5_at 87.5 P 0.000169
AFFX-PheX-M_at 117.7 P 0.010317
AFFX-PheX-3_at 134 P 0.033677
AFFX-ThrX-5_at 78.3 P 0.020022
AFFX-ThrX-M_at 155.6 P 0.000857

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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