|
| Status |
Public on Oct 20, 2014 |
| Title |
NC4 |
| Sample type |
RNA |
| |
|
| Source name |
demographically-matched controls
|
| Organism |
Homo sapiens |
| Characteristics |
blood: peripheral blood
average age: 41.42 ± 9.72
gender: 6 female/6 male
|
| Treatment protocol |
Mononuclear cells were isolated within 1 h by density-gradient centrifugation and stored in Trizol at − 80 °C.
|
| Growth protocol |
Peripheral blood (10 ml) was obtained just before breakfast .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIZol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling of fragmented ds cDNA according to the standard Affymetrix protocol (GeneChip® Eukaryotic Double Strand Whole Transcript Protocol, Affymetrix).
|
| |
|
| Hybridization protocol |
20 ug of Fragmented-labeled ds DNA were hybridized for for 16 hr at 45°C . Wash/Stain arrays with Affymetrix® Hybridization, Wash and Stain Kit using the FS450_0001 fluidics protocol.
|
| Scan protocol |
After hybridization and washing, processed slides were scanned with the Affymetrix GeneChip Scanner 3000 7G (Affymetrix, Santa Clara, CA).
|
| Data processing |
All gene level files were imported into Affymetrix Expression Console (version 1.2.1) and normalized by the quantile method. Combat Software was used to adjust the normalized intensity to remove batch effects.
|
| |
|
| Submission date |
Nov 27, 2013 |
| Last update date |
Oct 20, 2014 |
| Contact name |
Zhifen Liu |
| E-mail(s) |
liuzhifen5518@163.com
|
| Phone |
+863514639641
|
| Organization name |
1st hospital of Shanxi Medical University
|
| Department |
Psychiatry
|
| Street address |
85 Jiefang South Road
|
| City |
TaiYuan |
| State/province |
Shanxi |
| ZIP/Postal code |
030001 |
| Country |
China |
| |
|
| Platform ID |
GPL17976 |
| Series (1) |
| GSE52790 |
lncRNA and mRNA expression data in peripheral blood sampled from MDD patients |
|
