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Sample GSM1276035 Query DataSets for GSM1276035
Status Public on Oct 20, 2014
Title ND 10
Sample type RNA
 
Source name the patient in the first edisode of MDD
Organism Homo sapiens
Characteristics blood: peripheral blood
average age: 43.17 ± 8.66
gender: 5 female/5 male
Treatment protocol Mononuclear cells were isolated within 1 h by density-gradient centrifugation and stored in Trizol at − 80 °C.
Growth protocol Peripheral blood (10 ml) was obtained just before breakfast .
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIZol according to the manufacturer's instructions.
Label biotin
Label protocol Labeling of fragmented ds cDNA according to the standard Affymetrix protocol (GeneChip® Eukaryotic Double Strand Whole Transcript Protocol, Affymetrix).
 
Hybridization protocol 20 ug of Fragmented-labeled ds DNA were hybridized for for 16 hr at 45°C . Wash/Stain arrays with Affymetrix® Hybridization, Wash and Stain Kit using the FS450_0001 fluidics protocol.
Scan protocol After hybridization and washing, processed slides were scanned with the Affymetrix GeneChip Scanner 3000 7G (Affymetrix, Santa Clara, CA).
Data processing All gene level files were imported into Affymetrix Expression Console (version 1.2.1) and normalized by the quantile method. Combat Software was used to adjust the normalized intensity to remove batch effects.
 
Submission date Nov 27, 2013
Last update date Oct 20, 2014
Contact name Zhifen Liu
E-mail(s) liuzhifen5518@163.com
Phone +863514639641
Organization name 1st hospital of Shanxi Medical University
Department Psychiatry
Street address 85 Jiefang South Road
City TaiYuan
State/province Shanxi
ZIP/Postal code 030001
Country China
 
Platform ID GPL17976
Series (1)
GSE52790 lncRNA and mRNA expression data in peripheral blood sampled from MDD patients

Data table header descriptions
ID_REF
VALUE Combat Software-normalized data

Data table
ID_REF VALUE
TC0100001 5.17338
TC0100002 4.8152
TC0100003 7.40123
TC0100004 3.18339
TC0100005 3.44663
TC0100006 10.13411
TC0100008 3.14023
TC0100009 2.07414
TC0100010 0.84104
TC0100011 8.67591
TC0100012 7.11933
TC0100013 3.5837
TC0100014 5.18047
TC0100015 4.74158
TC0100016 4.5851
TC0100017 5.18171
TC0100018 1.71487
TC0100019 5.70909
TC0100020 5.628
TC0100021 5.9193

Total number of rows: 74057

Table truncated, full table size 1755 Kbytes.




Supplementary file Size Download File type/resource
GSM1276035_ND106_hGlue_3_0_v1_.CEL.gz 22.6 Mb (ftp)(http) CEL
GSM1276035_ND106_hGlue_3_0_v1_.rma-gene-default.chp.gz 318.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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