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Sample GSM1275155 Query DataSets for GSM1275155
Status Public on Feb 05, 2014
Title H1299_Ad-p73b
Sample type RNA
Source name H1299_Ad-p73b
Organism Homo sapiens
Characteristics cell line: H1299
cell type: human lung carcinoma cells
infected with: adenoviruses expressing p73b
Treatment protocol H1299 cells were infected with adenoviruses expressing LacZ, p53, p63a, p63g, p73a or p73b at 25 moi.
Growth protocol H1299 were cultured in RPMI1640 medium with 10% FCS.
Extracted molecule total RNA
Extraction protocol 24h after infection, total RNA was extracted by RNeasy mini kit (QIAGEN) according to manufacturer's protocol. RNA was quantified using the NanoDrop spectrophotometer.
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description Gene expression after Ad-p73b infection
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
The values in the data matrix (log2) were calculated by Limma (R package) for the normalization using all probes and then the values for lincRNAs were selected for further analysis.
Submission date Nov 26, 2013
Last update date Feb 05, 2014
Contact name Masashi Idogawa
Organization name Sapporo Medical University
Street address S1W17
City Sapporo
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL17077
Series (1)
GSE52739 The effect on lincRNA expression by p53 family members.

Data table header descriptions
VALUE log2 normalized

Data table
A_23_P117082 11.01010729
A_33_P3246448 5.774319787
A_33_P3318220 5.528653648
A_33_P3236322 5.802581817
A_33_P3319925 6.051189077
A_21_P0000509 15.79746677
A_21_P0000744 10.32049348
A_24_P215804 8.537132202
A_23_P110167 5.73586985
A_33_P3211513 6.609466451
A_23_P103349 5.237015967
A_32_P61480 5.528653648
A_33_P3788124 5.780886837
A_33_P3414202 6.460433629
A_33_P3316686 6.892526271
A_33_P3300975 9.282650933
A_33_P3263061 9.580530818
A_33_P3261373 5.802581817
A_24_P278460 6.659084514
A_21_P0013109 5.465003137

Total number of rows: 50599

Table truncated, full table size 1256 Kbytes.

Supplementary file Size Download File type/resource
GSM1275155_H1299_6.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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