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Sample GSM1275154 Query DataSets for GSM1275154
Status Public on Feb 05, 2014
Title H1299_Ad-p73a
Sample type RNA
Source name H1299_Ad-p73a
Organism Homo sapiens
Characteristics cell line: H1299
cell type: human lung carcinoma cells
infected with: adenoviruses expressing p73a
Treatment protocol H1299 cells were infected with adenoviruses expressing LacZ, p53, p63a, p63g, p73a or p73b at 25 moi.
Growth protocol H1299 were cultured in RPMI1640 medium with 10% FCS.
Extracted molecule total RNA
Extraction protocol 24h after infection, total RNA was extracted by RNeasy mini kit (QIAGEN) according to manufacturer's protocol. RNA was quantified using the NanoDrop spectrophotometer.
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description Gene expression after Ad-p73a infection
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
The values in the data matrix (log2) were calculated by Limma (R package) for the normalization using all probes and then the values for lincRNAs were selected for further analysis.
Submission date Nov 26, 2013
Last update date Feb 05, 2014
Contact name Masashi Idogawa
Organization name Sapporo Medical University
Street address S1W17
City Sapporo
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL17077
Series (1)
GSE52739 The effect on lincRNA expression by p53 family members.

Data table header descriptions
VALUE log2 normalized

Data table
A_23_P117082 11.47633474
A_33_P3246448 6.012609012
A_33_P3318220 5.834511144
A_33_P3236322 5.732006342
A_33_P3319925 5.902382252
A_21_P0000509 16.06100676
A_21_P0000744 11.02961701
A_24_P215804 8.864117426
A_23_P110167 5.548592902
A_33_P3211513 6.730451625
A_23_P103349 5.343948463
A_32_P61480 5.752202222
A_33_P3788124 5.688424853
A_33_P3414202 6.709352275
A_33_P3316686 7.142731366
A_33_P3300975 9.141943979
A_33_P3263061 9.930088478
A_33_P3261373 5.809016381
A_24_P278460 6.765682422
A_21_P0013109 5.732006342

Total number of rows: 50599

Table truncated, full table size 1254 Kbytes.

Supplementary file Size Download File type/resource
GSM1275154_H1299_5.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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