NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1274412 Query DataSets for GSM1274412
Status Public on Nov 25, 2013
Title 786-O_overexpression 1_rep3
Sample type RNA
 
Source name clear cell renal cell carcinoma cell line 786-O, stable transfection with overexpression of S100A6
Organism Homo sapiens
Characteristics cell line: clear cell renal cell carcinoma cell line 786-O
genotype/variation: overexpression of S100A6
Treatment protocol The 786-O cells were stable transfection with overexpression and knockdown of S100A6, each treatment had a vector transfection control. The final purity of stable transfection is 99%.
Growth protocol The 786-O cells were cultured in RPMI 1640 medium (HyClone), and the culture medium was supplemented with 10% fatal bovin serum (FBS, Gibco). The cells were maintained at 37℃ with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA from each sample was extracted using TRK1001(LC Sciences, Cat. TRK-1001, Total RNA Purification Kit)according to the manufacturer’s instructions. Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific) and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).
Label cy3
Label protocol The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP.
 
Hybridization protocol The labeled cRNAs were hybridized onto the microarray according to the manufacturer’s standard protocols.
Scan protocol After washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies).
Description Sample name: OE1
Total 344 different expression genes (DEGs) including 191 up-regulated and 153 down regulated genes were found in overexpression S100A6 and vector control group.
Data processing Feature Extraction software (version10.7.1.1, Agilent Technologies) was used to analyze array images to get raw data. Genesrping were employed to finish the basic analysis with the raw data. To begin with, the raw data was normalized with the quantile algorithm. The probes that at least 1 out of all samples have flags in Detected were chosen for further data analysis. Differentially expressed genes were then identified through fold change as well as P value calculated with t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05. expression pattern among samples.
 
Submission date Nov 25, 2013
Last update date Apr 23, 2018
Contact name xu zhang
Organization name PLA general hospital
Street address Fuxing road
City Beijing
ZIP/Postal code 100853
Country China
 
Platform ID GPL17077
Series (1)
GSE52708 The mechanism of elevated S100A6 (Calcyclin) Enhances Tumorigenesis in clear cell renal cell carcinoma
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE quantile-normalized signal intensity

Data table
ID_REF VALUE
A_23_P117082 13.32
A_33_P3246448 5.57
A_33_P3318220 2.37
A_33_P3236322 2.36
A_33_P3319925 3.31
A_21_P0000509 16.64
A_21_P0000744 11.66
A_24_P215804 8.32
A_23_P110167 10.97
A_33_P3211513 7.07
A_23_P103349 2.27
A_32_P61480 2.84
A_33_P3788124 2.33
A_33_P3414202 6.39
A_33_P3316686 8.32
A_33_P3300975 10.21
A_33_P3263061 11.73
A_33_P3261373 2.31
A_24_P278460 8.23
A_21_P0013109 2.31

Total number of rows: 50737

Table truncated, full table size 925 Kbytes.




Supplementary file Size Download File type/resource
GSM1274412_OE1.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap