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Sample GSM1273246 Query DataSets for GSM1273246
Status Public on Mar 01, 2015
Title tail-1414
Sample type RNA
 
Source name tail skin
Organism Mus musculus
Characteristics tissue: tail skin
age: 8 weeks
hras_genotype: KO
plate: 1
mouse.no: 1414
microarray.outlier: no
Sex: F
Treatment protocol Mice were aged to 8 weeks, untreated
Growth protocol Mice were housed in standard conditions with standard chow and water ad libitum
Extracted molecule total RNA
Extraction protocol Trizol followed by Qiagen RNEasy clean-up
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA
 
Hybridization protocol 90 microliters of hybridization sample were incubated in the Affymetrix GeneTitan Hybridization Oven instrument for 16 hours at 48C
Scan protocol Arrays were processed with the Affymetrix GeneTitan Fluidics Station instrument, using the MoGene-1_1-st-v1-96 fluidics script and GeneTitan Hybridization, Wash, and Stain Kit for WT Array Plates. Arrays were scanned in the Affymetrix GeneTitan Multi-Channel (MC) instrument with the AGCC GeneTitan Instrument Control.
Description Gene expression data from untreated P56 mouse skin
Data processing CEL files were background corrected and normalized with RMA using oligo in R and a custom probe database which avoids SNPs in FVB and Spretus genomes. Data were batch-corrected for plate and genotype and then log2 transformed.
 
Submission date Nov 21, 2013
Last update date Mar 01, 2015
Contact name David Quigley
Organization name UCSF
Department Helen Diller Comprehensive Cancer Center
Lab Ashworth Lab
Street address 1450 Third St. Room 207
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL11533
Series (1)
GSE60769 Normal mouse skin lacking Hras

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
10344614 4.578
10344616 1.648
10344620 2.296
10344622 4.254
10344624 9.142
10344633 7.958
10344637 8.466
10344653 2.721
10344658 8.202
10344674 2.249
10344679 2.708
10344707 8.606
10344713 7.528
10344715 2.607
10344717 1.945
10344719 3.691
10344721 1.603
10344723 4.517
10344725 5.101
10344741 9.716

Total number of rows: 35556

Table truncated, full table size 517 Kbytes.




Supplementary file Size Download File type/resource
GSM1273246_Balmain_Aim2_U01_P1_H08_1414.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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