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Sample GSM127043 Query DataSets for GSM127043
Status Public on Sep 01, 2006
Title Androgen-starved LNCaP cells, treated with 1nM R1881 and DMSO for 6h, biol repl 2 (gedunin)
Sample type RNA
Source name LNCaP cell line
Organism Homo sapiens
Characteristics androgen-starved LNCaP cells, treated with 1nM R1881 and DMSO for 6h
Treatment protocol LNCaP cells were grown in RPMI and charcol-stripped 10%FBS for 2d and then treated with 1nM R1881 and DMSO for 24h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained on a Affymetrix Fluidics station
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner
Description Gene expression data from androgen-starved LNCaP cells treated with 1nM R1881 and DMSO for 24h
Data processing The data were processed by RMA (GenePattern)
Submission date Aug 11, 2006
Last update date Aug 11, 2006
Contact name Haley Hieronymus
Organization name Broad Institute of Harvard and MIT
Street address 7 Cambridge Center
State/province MA
ZIP/Postal code 02142
Country USA
Platform ID GPL571
Series (1)
GSE5508 Androgen-starved LNCaP cells treated with gedunin and androgen, androgen alone, or vehicle alone for 24h

Data table header descriptions
VALUE RMA-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
1007_s_at 1048.599 P
1053_at 70.021 P
117_at 32.456 A
121_at 293.453 P
1255_g_at 15.48 A
1294_at 59.85 A
1316_at 52.169 P
1320_at 31.965 A
1405_i_at 12.88 A
1431_at 14.44 A
1438_at 100.076 A
1487_at 159.252 P
1494_f_at 50.457 A
1598_g_at 255.663 P
160020_at 174.342 P
1729_at 162.118 P
1773_at 30.325 A
177_at 22.912 A
179_at 632.333 P
1861_at 78.839 P

Total number of rows: 22277

Table truncated, full table size 439 Kbytes.

Supplementary data files not provided

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