|
| Status |
Public on Nov 13, 2013 |
| Title |
SSC rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Spermatogonial Stem Cells from testis replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: cultured cells
gender: male (XY)
cell type: Spermatogonial Stem Cells from testis
|
| Treatment protocol |
hESCs cultured in EB medium for 3 days and re-cultured for 4 additional days in EB medium with BMP4 and RA to specify PGC-like cells. The resultant cells were cultured in germ cell (GC)-proliferation medium for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each total RNA sample (200 ng) obtained from the ESC-derived PGC-like cells and testis-derived SSCs were labeled and amplified using the Low Input Quick Amp Labeling kit (Agilent Technologies, Palo Alto, CA).
|
| Label |
Cy3
|
| Label protocol |
The Cy3-labeled RNAs were resuspended in 50 μl of hybridization solution (Agilent Technologies).
|
| |
|
| Hybridization protocol |
After the labeled RNAs were placed in an Agilent SurePrint G3 Human Gene Expression 8x60K Microarray (Agilent Technologies) and covered with a Gasket 8-plex slide (Agilent Technologies), the slides were hybridized for 17 hr at 65°C.
|
| Scan protocol |
The arrays were analyzed on an Agilent scanner using the associated software.
|
| Description |
Gene expression after 14 days cultured SSCs set 1
|
| Data processing |
The gene expression levels were calculated using Feature Extraction v10.7.3.1 (Agilent Technologies).
|
| |
|
| Submission date |
Nov 12, 2013 |
| Last update date |
Nov 13, 2013 |
| Contact name |
LIM JUNG JIN |
| E-mail(s) |
limjj7657@hanmail.net
|
| Organization name |
CHA University
|
| Department |
Fertility Center of CHA Gangnam Medical Center
|
| Street address |
606-5 Yeoksam-dong, Gangnam-gu
|
| City |
Seoul |
| ZIP/Postal code |
135-081 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE52302 |
Comparison of gene expression (hESC, PGC-like cell from hESC, SSC from testis) |
|
