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Sample GSM1262665 Query DataSets for GSM1262665
Status Public on Nov 13, 2013
Title PGC-like cell rep 2
Sample type RNA
Source name PGC-like cells from undifferentiated hESC replicate 2
Organism Homo sapiens
Characteristics sample type: cultured cells
gender: male (XY)
cell type: PGC-like cells from undifferentiated hESC
Treatment protocol hESCs cultured in EB medium for 3 days and re-cultured for 4 additional days in EB medium with BMP4 and RA to specify PGC-like cells. The resultant cells were cultured in germ cell (GC)-proliferation medium for 7 days.
Extracted molecule total RNA
Extraction protocol Each total RNA sample (200 ng) obtained from the ESC-derived PGC-like cells and testis-derived SSCs were labeled and amplified using the Low Input Quick Amp Labeling kit (Agilent Technologies, Palo Alto, CA).
Label Cy3
Label protocol The Cy3-labeled RNAs were resuspended in 50 μl of hybridization solution (Agilent Technologies).
Hybridization protocol After the labeled RNAs were placed in an Agilent SurePrint G3 Human Gene Expression 8x60K Microarray (Agilent Technologies) and covered with a Gasket 8-plex slide (Agilent Technologies), the slides were hybridized for 17 hr at 65°C.
Scan protocol The arrays were analyzed on an Agilent scanner using the associated software.
Description Gene expression after 14 days treatment protocol set 2
Data processing The gene expression levels were calculated using Feature Extraction v10.7.3.1 (Agilent Technologies).
Submission date Nov 12, 2013
Last update date Nov 13, 2013
Contact name LIM JUNG JIN
Organization name CHA University
Department Fertility Center of CHA Gangnam Medical Center
Street address 606-5 Yeoksam-dong, Gangnam-gu
City Seoul
ZIP/Postal code 135-081
Country South Korea
Platform ID GPL13607
Series (1)
GSE52302 Comparison of gene expression (hESC, PGC-like cell from hESC, SSC from testis)

Data table header descriptions
VALUE gProcessedSignal

Data table
1 1.01E+05
2 2.07E+01
3 1.96E+01
4 4.31E+02
5 9.56E+02
6 8.86E+01
7 5.30E+03
8 1.28E+03
9 1.91E+01
10 4.40E+01
11 1.90E+01
12 1.37E+03
13 1.57E+03
14 5.24E+02
15 5.67E+03
16 1.86E+01
17 1.07E+02
18 1.84E+01
19 2.56E+02
20 8.56E+02

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM1262665_S01_GE1_107_Sep09_2_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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