|
| Status |
Public on Dec 02, 2013 |
| Title |
RN2_TRMPV_Brg1sh_3364_dox_96h_2 |
| Sample type |
RNA |
| |
|
| Source name |
MLL-AF9/NrasG12D murine cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line background: RN2
cell line: MLL-AF9/NrasG12D
cell type: Acute myeloid leukemia cells (AML)
transfected with: TRMPV_Brg1sh (shRNA targeting Brg1)
treated with: 1ug/ml doxycycline for 96h
|
| Treatment protocol |
Before RNA preparation, murine AML cell lines (RN2) transduced with indicated constructs were treated with 1ug/ml doxycycline to induce shRNA expression for 96h. TRMPV_Rluc is negative control shRNA targeting Renilla luciferase.
|
| Growth protocol |
Murine AML cell lines (RN2) transduced with indicated constructs were cultured in RPMI1640 supplement with 10% FBS and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated by using RNeasy Mini Kit (QIAGEN, #74104)
|
| Label |
Biotin
|
| Label protocol |
The cDNA was then fragmented and terminally labeled with biotin, using the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
according to the manufacturer's instructions on Mouse Gene ST 1.0 GeneChips
|
| Scan protocol |
according to the manufacturer's instructions on Mouse Gene ST 1.0 GeneChips
|
| Data processing |
Data was processed by RMA in R software
|
| |
|
| Submission date |
Nov 08, 2013 |
| Last update date |
Dec 02, 2013 |
| Contact name |
Christopher R Vakoc |
| Organization name |
Cold Spring Harbor Lab
|
| Lab |
Vakoc Lab
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE52231 |
Role of SWI/SNF in acute leukemia maintenance and enhancer-mediated Myc regulation [array] |
| GSE52279 |
Role of SWI/SNF in acute leukemia maintenance and enhancer-mediated Myc regulation |
|
