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Sample GSM1255316 Query DataSets for GSM1255316
Status Public on Dec 01, 2015
Title genomic DNA from fibroblasts FIB-SP-17-CON
Sample type genomic
Source name IPSC-control
Organism Homo sapiens
Characteristics patient id: SP-17
patient id2: C-04
gender: male
disease state: Healthy
number of ipsc obtained to generate neurons: NA
family history of pd: no
age at onset (years): NA
age at tissue collection (years): 52
l-dopa response: NA
initial symptoms: NA
cell type: fibroblasts
disease state: control
Treatment protocol No specific treatment of cells
Growth protocol Keratinocytes or dermal fibroblasts were cultured using standard protocols from skin biopsy obtained from participating subjects [Sanchez-Danes et al., 2012]. Briefly, keratinocytes and/ or fibroblasts were transformed to induced pluripotent stem cells (iPSC) by retroviral transduction of three transcription factors (OTC4, SOX2 and KLF4). Subsequently, iPSC were differentiated into DAn specific growth factor medium supplementation (bFGF, FGF-8 and SHH) and lentiviral LMX1A delivery.Described in Sanchez-Danes et al., EMBO Mol Med. 2012 ;4(5):380-95. doi: 10.1002/emmm.201200215.
Extracted molecule genomic DNA
Extraction protocol DNA extracted according to AllPrep DNA/RNA/Protein Mini Kit - Qiagen
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
Scan protocol Arrays were imaged using the iScan reader using standard recommended Illumina scanner setting
Data processing Using the minfi R package available through Bioconductor
Submission date Oct 30, 2013
Last update date Dec 01, 2015
Contact name Mario Ezquerra
Phone 0034 932273122
Organization name Fundacio Clinic/IDIBAPS
Department Neurology
Lab Neurodegenerative Diseases
Street address Villarroel 170
City Barcelona
State/province Catalonia
ZIP/Postal code 08027
Country Spain
Platform ID GPL13534
Series (2)
GSE51921 DNA methylation analysis in idiopathic and LRRK2-associated Parkinson's disease (PD)
GSE51923 Idiopathic and LRRK2-associated Parkinson's disease

Data table header descriptions
VALUE Average beta values

Data table
cg00014152 0.821195145
cg00021786 0.945220419
cg00072839 0.03456906
cg00074638 0.534970666
cg00266918 0.091713881
cg00378717 0.029753687
cg00378950 0.451031656
cg00381376 0.028637413
cg00388637 0.623235613
cg00423014 0.642847193
cg00457389 0.045667991
cg00551957 0.799007288
cg00573298 0.039415589
cg00685229 0.795016988
cg00697812 0.755830224
cg00751785 0.038322333
cg00812634 0.941569256
cg00824273 0.680073595
cg00824714 0.66309987
cg00839572 0.055049558

Total number of rows: 478178

Table truncated, full table size 10698 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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