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Sample GSM1255313 Query DataSets for GSM1255313
Status Public on Dec 01, 2015
Title genomic DNA from IPSC IPSC.SP-13-L2PD
Sample type genomic
Source name IPSC-PD LRRK2 G2019S mutation carrier
Organism Homo sapiens
Characteristics patient id: SP-13
patient id2: PD-01
gender: female
disease state: Parkinson's disease
number of ipsc obtained to generate neurons: NA
family history of pd: yes
age at onset (years): 57
age at tissue collection (years): 68
l-dopa response: good
initial symptoms: tremor
cell type: IPSC
disease state: Parkinson's disease
Treatment protocol No specific treatment of cells
Growth protocol Keratinocytes or dermal fibroblasts were cultured using standard protocols from skin biopsy obtained from participating subjects [Sanchez-Danes et al., 2012]. Briefly, keratinocytes and/ or fibroblasts were transformed to induced pluripotent stem cells (iPSC) by retroviral transduction of three transcription factors (OTC4, SOX2 and KLF4). Subsequently, iPSC were differentiated into DAn specific growth factor medium supplementation (bFGF, FGF-8 and SHH) and lentiviral LMX1A delivery.Described in Sanchez-Danes et al., EMBO Mol Med. 2012 ;4(5):380-95. doi: 10.1002/emmm.201200215.
Extracted molecule genomic DNA
Extraction protocol DNA extracted according to AllPrep DNA/RNA/Protein Mini Kit - Qiagen
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
Scan protocol Arrays were imaged using the iScan reader using standard recommended Illumina scanner setting
Description 8918692127_R03C02
Data processing Using the minfi R package available through Bioconductor
Submission date Oct 30, 2013
Last update date Dec 01, 2015
Contact name Mario Ezquerra
Phone 0034 932273122
Organization name Fundacio Clinic/IDIBAPS
Department Neurology
Lab Neurodegenerative Diseases
Street address Villarroel 170
City Barcelona
State/province Catalonia
ZIP/Postal code 08027
Country Spain
Platform ID GPL13534
Series (2)
GSE51921 DNA methylation analysis in idiopathic and LRRK2-associated Parkinson's disease (PD)
GSE51923 Idiopathic and LRRK2-associated Parkinson's disease

Data table header descriptions
VALUE Average beta values

Data table
cg00014152 0.932406334
cg00021786 0.922137987
cg00072839 0.02778948
cg00074638 0.603273263
cg00266918 0.191709229
cg00378717 0.345940933
cg00378950 0.881619617
cg00381376 0.427549883
cg00388637 0.708584987
cg00423014 0.676042677
cg00457389 0.096051227
cg00551957 0.751889862
cg00573298 0.528579191
cg00685229 0.476518783
cg00697812 0.79912459
cg00751785 0.58540375
cg00812634 0.943539559
cg00824273 0.713616276
cg00824714 0.77670669
cg00839572 0.285134133

Total number of rows: 478178

Table truncated, full table size 10697 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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