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Sample GSM1255296 Query DataSets for GSM1255296
Status Public on Dec 01, 2015
Title genomic DNA from IPSC-derived DAn SP-17-CON
Sample type genomic
Source name 30 days-neuron control
Organism Homo sapiens
Characteristics patient id: SP-17
patient id2: C-04
gender: male
disease state: Healthy
number of ipsc obtained to generate neurons: 3
family history of pd: no
age at onset (years): NA
age at tissue collection (years): 52
l-dopa response: NA
initial symptoms: NA
cell type: IPSC-derived DAn
disease state: control
Treatment protocol No specific treatment of cells
Growth protocol Keratinocytes or dermal fibroblasts were cultured using standard protocols from skin biopsy obtained from participating subjects [Sanchez-Danes et al., 2012]. Briefly, keratinocytes and/ or fibroblasts were transformed to induced pluripotent stem cells (iPSC) by retroviral transduction of three transcription factors (OTC4, SOX2 and KLF4). Subsequently, iPSC were differentiated into DAn specific growth factor medium supplementation (bFGF, FGF-8 and SHH) and lentiviral LMX1A delivery.Described in Sanchez-Danes et al., EMBO Mol Med. 2012 ;4(5):380-95. doi: 10.1002/emmm.201200215.
Extracted molecule genomic DNA
Extraction protocol DNA extracted according to AllPrep DNA/RNA/Protein Mini Kit - Qiagen
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
Scan protocol Arrays were imaged using the iScan reader using standard recommended Illumina scanner setting
Description 7990895162_R04C01
Data processing Using the minfi R package available through Bioconductor
Submission date Oct 30, 2013
Last update date Dec 01, 2015
Contact name Mario Ezquerra
Phone 0034 932273122
Organization name Fundacio Clinic/IDIBAPS
Department Neurology
Lab Neurodegenerative Diseases
Street address Villarroel 170
City Barcelona
State/province Catalonia
ZIP/Postal code 08027
Country Spain
Platform ID GPL13534
Series (2)
GSE51921 DNA methylation analysis in idiopathic and LRRK2-associated Parkinson's disease (PD)
GSE51923 Idiopathic and LRRK2-associated Parkinson's disease

Data table header descriptions
VALUE Average beta values

Data table
cg00014152 0.967694994
cg00021786 0.957931287
cg00072839 0.057090583
cg00074638 0.586670127
cg00266918 0.101750547
cg00378717 0.011249343
cg00378950 0.887022516
cg00381376 0.016560446
cg00388637 0.673782405
cg00423014 0.679243697
cg00457389 0.035361414
cg00551957 0.916732335
cg00573298 0.02321329
cg00685229 0.468932122
cg00697812 0.806484817
cg00751785 0.041085881
cg00812634 0.975090064
cg00824273 0.703740088
cg00824714 0.849588011
cg00839572 0.035741092

Total number of rows: 478178

Table truncated, full table size 10697 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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