|
Status |
Public on Oct 29, 2013 |
Title |
parental DU145 cells |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
parental DU145 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: DU145 cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purfied using Trizol followed by Rneasy MinElute clean up kit, according to manufacturers instructions
|
Label |
Hy3
|
Label protocol |
0.5ug RNA labeled using miRCURY miRNA array Power labeling kit (Exiqon, Denmark)
|
|
|
Channel 2 |
Source name |
Common reference
|
Organism |
Homo sapiens |
Characteristics |
reference: Reference pool made from all samples
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purfied using Trizol followed by Rneasy MinElute clean up kit, according to manufacturers instructions
|
Label |
Hy5
|
Label protocol |
0.5ug RNA labeled using miRCURY miRNA array Power labeling kit (Exiqon, Denmark)
|
|
|
|
Hybridization protocol |
Hybridized to miRCURY LNA array version 11.0 (Exiqon, Denmark) according to array manual using Tecan HS4800 hybridization station (Tecan, Austria)
|
Scan protocol |
scanned using Agilent G2565BA Microarray scanner system (Agilent Technologies), image analysis using ImaGene 8.0 software (BioDiscovery Inc, USA)
|
Description |
Common reference ppol
|
Data processing |
LOESS normalized, background correction using Normexp with offset value 10 (Convolution model from Richie, ME, Bioinformatics, 2007, Vol 23 , no 20, 2700-2707)
|
|
|
Submission date |
Oct 27, 2013 |
Last update date |
Oct 29, 2013 |
Contact name |
Jacqueline Banyard |
Organization name |
Boston Childrens Hospital
|
Department |
Surgery
|
Lab |
Vascular Biology Division
|
Street address |
300 Longwood Ave
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL7723 |
Series (2) |
GSE51753 |
MicroRNA expression in DU145 sublines metastatic to lymph nodes |
GSE51756 |
Gene and miRNA expression data in the DU145-LN metastatic cell series |
|