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Sample GSM1252002 Query DataSets for GSM1252002
Status Public on Oct 29, 2013
Title parental DU145 cells
Sample type RNA
 
Channel 1
Source name parental DU145 cells
Organism Homo sapiens
Characteristics cell line: DU145 cells
Extracted molecule total RNA
Extraction protocol Total RNA was purfied using Trizol followed by Rneasy MinElute clean up kit, according to manufacturers instructions
Label Hy3
Label protocol 0.5ug RNA labeled using miRCURY miRNA array Power labeling kit (Exiqon, Denmark)
 
Channel 2
Source name Common reference
Organism Homo sapiens
Characteristics reference: Reference pool made from all samples
Extracted molecule total RNA
Extraction protocol Total RNA was purfied using Trizol followed by Rneasy MinElute clean up kit, according to manufacturers instructions
Label Hy5
Label protocol 0.5ug RNA labeled using miRCURY miRNA array Power labeling kit (Exiqon, Denmark)
 
 
Hybridization protocol Hybridized to miRCURY LNA array version 11.0 (Exiqon, Denmark) according to array manual using Tecan HS4800 hybridization station (Tecan, Austria)
Scan protocol scanned using Agilent G2565BA Microarray scanner system (Agilent Technologies), image analysis using ImaGene 8.0 software (BioDiscovery Inc, USA)
Description Common reference ppol
Data processing LOESS normalized, background correction using Normexp with offset value 10 (Convolution model from Richie, ME, Bioinformatics, 2007, Vol 23 , no 20, 2700-2707)
 
Submission date Oct 27, 2013
Last update date Oct 29, 2013
Contact name Jacqueline Banyard
Organization name Boston Childrens Hospital
Department Surgery
Lab Vascular Biology Division
Street address 300 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL7723
Series (2)
GSE51753 MicroRNA expression in DU145 sublines metastatic to lymph nodes
GSE51756 Gene and miRNA expression data in the DU145-LN metastatic cell series

Data table header descriptions
ID_REF
VALUE log2 ratio (Hy3/Hy5) representing sample/common reference pool. Replicated measurements on the same slide have been averaged. Spots with flags 1 or higher have been removed

Data table
ID_REF VALUE
17748 -0.16
17888 0.29
17749 -0.17
42769 -0.05
19004 -0.10
42653 null
46437 -0.30
42505 null
46436 -0.11
42743 null
46435 -0.29
42768 null
42742 null
46438 -0.21
42778 null
9938 0.05
42534 null
10916 null
19581 -0.28
42563 null

Total number of rows: 1383

Table truncated, full table size 15 Kbytes.




Supplementary file Size Download File type/resource
GSM1252002_0_Exiqon_13973561_S01_Cropped.txt.gz 779.3 Kb (ftp)(http) TXT
GSM1252002_1_Exiqon_13973561_S01_Cropped.txt.gz 865.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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