|
| Status |
Public on Oct 26, 2013 |
| Title |
PSM05_LN4D6 CREB3L1 cells |
| Sample type |
RNA |
| |
|
| Source name |
LN4D6 CREB3L1 cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: LN4D6
cell type: rat mammary tumor cells
tansfected with: CREB3L1
|
| Growth protocol |
Cells were grown in 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNA extraction kit according to manufacturers instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotin labelling was prepared using the 3' IVT Express Kit according to manufacturers instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized and stained using the Genechip hybridization, stain and wash kit according to manufacturers instructions.
|
| Scan protocol |
Gene chips were scanned using Genechip Scanner 3000 7G
|
| Description |
Gene expression data from LN4D6 CREB3L1 cells
|
| Data processing |
The data was analyzed with Partek Genomics Suite v6.6 using RMA (data in log2)
|
| |
|
| Submission date |
Oct 25, 2013 |
| Last update date |
Oct 29, 2013 |
| Contact name |
Deborah Anderson |
| E-mail(s) |
deborah.anderson@saskcancer.ca
|
| Phone |
13069667038
|
| Organization name |
University of Saskatchewan
|
| Street address |
107 Wiggins Road
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N 5E5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE51708 |
Effects of CREB3L1 on gene expression |
| GSE51857 |
CREB3L1 is a metastasis suppressor that represses expression of genes regulating metastasis, invasion and angiogenesis |
|
