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Sample GSM1246570 Query DataSets for GSM1246570
Status Public on Nov 30, 2013
Title J1 Thoc5 siRNA_rep2
Sample type RNA
 
Source name mouse J1 ESC
Organism Mus musculus
Characteristics cell type: embryonic cell line
cell line: J1
transfection: Thoc5 siRNA
Treatment protocol Cell were transfected with siRNAs against NT, Thoc2 and Thoc5. Total RNA was harvested 96 hours after transfection.
Growth protocol J1 cell were maintianed in ESGRO medium. Cell were cultured in M10 medium when transfection or infection was performed.
Extracted molecule total RNA
Extraction protocol Total RNA were prepared using Therno RNA kit according to the manufacturer's instructions.
Label Cy3
Label protocol Labeling was carried out with the Agilent Low Input Quick Amp Labeling Kit, one color, using 1 ug total RNA.
 
Hybridization protocol Hybridization was carried out on Agilent Mouse GE 4x44K v2 Microarray (G2519F) accrording to Agilent protocol.
Scan protocol Arrays were scanned on Agilent SureScan Microarray Scanner according to manufacturer's instructions using the one color scan setting.
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 11.0 using default parameters to obtain background subtracted Processed Signal intensities.
 
Submission date Oct 21, 2013
Last update date Nov 30, 2013
Contact name Li Wang
Organization name NIH
Street address 111 T.W. Alexander Dr
City Durham
ZIP/Postal code 27709
Country USA
 
Platform ID GPL4134
Series (2)
GSE51496 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (microarray)
GSE51534 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 14.1846192896239
2 2.00945070451475
3 2.01497886362619
4 2.01922051223704
5 2.02241108688915
6 2.02481006959753
7 2.02650129823572
8 2.02732876518865
9 2.02788156135729
10 2.02797967873845
11 2.02772008145099
12 2.02693162814348
13 5.84190673517517
14 2.02454911613508
15 2.35532179541577
16 9.42369354799768
18 2.01361648784974
19 2.00986752263258
20 2.00702717716366
21 4.01724932049661

Total number of rows: 45018

Table truncated, full table size 995 Kbytes.




Supplementary file Size Download File type/resource
GSM1246570_Thoc5KD_rep2_US23502387_251486837643_S01_GE1-v5_95_Feb07_1_3.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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