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Sample GSM1246566 Query DataSets for GSM1246566
Status Public on Nov 30, 2013
Title J1 NT siRNA_rep2
Sample type RNA
Source name mouse J1 ESC
Organism Mus musculus
Characteristics cell type: embryonic cell line
cell line: J1
transfection: non-targeting siRNA
Treatment protocol Cell were transfected with siRNAs against NT, Thoc2 and Thoc5. Total RNA was harvested 96 hours after transfection.
Growth protocol J1 cell were maintianed in ESGRO medium. Cell were cultured in M10 medium when transfection or infection was performed.
Extracted molecule total RNA
Extraction protocol Total RNA were prepared using Therno RNA kit according to the manufacturer's instructions.
Label Cy3
Label protocol Labeling was carried out with the Agilent Low Input Quick Amp Labeling Kit, one color, using 1 ug total RNA.
Hybridization protocol Hybridization was carried out on Agilent Mouse GE 4x44K v2 Microarray (G2519F) accrording to Agilent protocol.
Scan protocol Arrays were scanned on Agilent SureScan Microarray Scanner according to manufacturer's instructions using the one color scan setting.
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 11.0 using default parameters to obtain background subtracted Processed Signal intensities.
Submission date Oct 21, 2013
Last update date Nov 30, 2013
Contact name Li Wang
Organization name NIH
Street address 111 T.W. Alexander Dr
City Durham
ZIP/Postal code 27709
Country USA
Platform ID GPL4134
Series (2)
GSE51496 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (microarray)
GSE51534 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 14.0083115984489
2 2.24620733821664
3 2.27182834166669
4 2.29398774606755
5 2.30980644913551
6 2.3203523888038
7 2.33399756931132
8 2.34549088516432
9 2.35398566159319
10 2.36483819586331
11 3.26870859977716
12 2.37822481947386
13 4.90911344933459
14 2.38869329995436
15 2.39305140097311
16 9.87652823813093
18 2.3997063056956
19 2.40141469756785
20 2.40162350428112
21 2.98590498656154

Total number of rows: 45018

Table truncated, full table size 995 Kbytes.

Supplementary file Size Download File type/resource
GSM1246566_NT_rep2_US23502387_251486837643_S01_GE1-v5_95_Feb07_1_2.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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