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Sample GSM1246565 Query DataSets for GSM1246565
Status Public on Nov 30, 2013
Title J1 NT siRNA_rep1
Sample type RNA
Source name mouse J1 ESC
Organism Mus musculus
Characteristics cell type: embryonic cell line
cell line: J1
transfection: non-targeting siRNA
Treatment protocol Cell were transfected with siRNAs against NT, Thoc2 and Thoc5. Total RNA was harvested 96 hours after transfection.
Growth protocol J1 cell were maintianed in ESGRO medium. Cell were cultured in M10 medium when transfection or infection was performed.
Extracted molecule total RNA
Extraction protocol Total RNA were prepared using Therno RNA kit according to the manufacturer's instructions.
Label Cy3
Label protocol Labeling was carried out with the Agilent Low Input Quick Amp Labeling Kit, one color, using 1 ug total RNA.
Hybridization protocol Hybridization was carried out on Agilent Mouse GE 4x44K v2 Microarray (G2519F) accrording to Agilent protocol.
Scan protocol Arrays were scanned on Agilent SureScan Microarray Scanner according to manufacturer's instructions using the one color scan setting.
Data processing The scanned images were analyzed with Agilent Feature Extraction Software 11.0 using default parameters to obtain background subtracted Processed Signal intensities.
Submission date Oct 21, 2013
Last update date Nov 30, 2013
Contact name Li Wang
Organization name NIH
Street address 111 T.W. Alexander Dr
City Durham
ZIP/Postal code 27709
Country USA
Platform ID GPL4134
Series (2)
GSE51496 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming (microarray)
GSE51534 The THO complex regulates pluripotency gene mRNA export to control embryonic stem cell self-renewal and somatic cell reprogramming

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 14.396041156572
2 1.94287389773555
3 1.96227326771118
4 1.98169700079546
5 2.00332880798248
6 2.02548938072957
7 2.04624490245945
8 2.06264054365187
9 2.07517040836729
10 2.0865665644164
11 2.09762497792153
12 2.10649769307456
13 6.23269981098114
14 4.47866967620004
15 3.53161163166791
16 9.98405888975345
18 4.22202962072774
19 2.14573502212039
20 3.03589382428374
21 2.5436668685371

Total number of rows: 45018

Table truncated, full table size 995 Kbytes.

Supplementary file Size Download File type/resource
GSM1246565_NT_rep1_US23502387_251486837642_S01_GE1-v5_95_Feb07_1_2.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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