GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1240944 Query DataSets for GSM1240944
Status Public on Dec 31, 2016
Title Human Mammary Epithelial Cells_GFP_Transfected_3
Sample type RNA
Source name HMECs_GFP_Transfected
Organism Homo sapiens
Characteristics cell type: Human Mammary Epithelial cells (HMECs)
genotype/variation: over-expressing GFP (control)
Treatment protocol Cells were brought to quisecence by serum starvation and treated with adenovirus to over-express the Rheb oncogene (10 replicates) or GFP (10 replicates). Eighteen hours after infection, activation of oncogenes and secondary targets were confirmed by western blotting.
Growth protocol Mammary epithelial cells were isolated from a breast reductive surgery at Duke University and grown at 37 degrees C in mammary epithelial cell growth medium (MEGM) containing a MEGM Bullet Kit, human transferrin and isopenterol.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a Qiagen Rneasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Gene Array Scanner (Affymetrix) following the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
Description 35214_GFP3
Data processing The data were analyzed using the R statistical platform with the Bioconductor affymetrix package [R version 2.13.1 (2011-07-08) and Bioconductor version 2.9].
Submission date Sep 27, 2013
Last update date Dec 31, 2016
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE51242 A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC]
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy

Data table header descriptions
VALUE Log2 GC-RMA signal.

Data table
1007_s_at 11.81093979
1053_at 6.315848185
117_at 9.010157711
121_at 7.486471833
1255_g_at 3.676403249
1294_at 7.012167606
1316_at 6.43752226
1320_at 5.58971164
1405_i_at 4.819608209
1431_at 4.067700753
1438_at 7.837947255
1487_at 8.179700593
1494_f_at 5.195493857
1598_g_at 11.25600133
160020_at 11.22257818
1729_at 8.627054772
177_at 6.39330649
1773_at 6.552675767
179_at 9.400635273
1861_at 7.014108507

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM1240944_2857_8676_35214_GFP3_HG-U133A+2.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap