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Sample GSM1240943 Query DataSets for GSM1240943
Status Public on Dec 31, 2016
Title Human Mammary Epithelial Cells_GFP_Transfected_2
Sample type RNA
Source name HMECs_GFP_Transfected
Organism Homo sapiens
Characteristics cell type: Human Mammary Epithelial cells (HMECs)
genotype/variation: over-expressing GFP (control)
Treatment protocol Cells were brought to quisecence by serum starvation and treated with adenovirus to over-express the Rheb oncogene (10 replicates) or GFP (10 replicates). Eighteen hours after infection, activation of oncogenes and secondary targets were confirmed by western blotting.
Growth protocol Mammary epithelial cells were isolated from a breast reductive surgery at Duke University and grown at 37 degrees C in mammary epithelial cell growth medium (MEGM) containing a MEGM Bullet Kit, human transferrin and isopenterol.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a Qiagen Rneasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Gene Array Scanner (Affymetrix) following the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
Description 35213_GFP2
Data processing The data were analyzed using the R statistical platform with the Bioconductor affymetrix package [R version 2.13.1 (2011-07-08) and Bioconductor version 2.9].
Submission date Sep 27, 2013
Last update date Dec 31, 2016
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE51242 A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC]
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy

Data table header descriptions
VALUE Log2 GC-RMA signal.

Data table
1007_s_at 11.89691952
1053_at 6.306613487
117_at 8.764534032
121_at 7.237157458
1255_g_at 3.660929503
1294_at 7.680666263
1316_at 6.427925367
1320_at 5.515904814
1405_i_at 5.072289905
1431_at 4.075292126
1438_at 8.068895606
1487_at 8.164582091
1494_f_at 4.864736113
1598_g_at 11.43647685
160020_at 11.22358255
1729_at 8.788538837
177_at 6.654510072
1773_at 6.506484361
179_at 9.308208051
1861_at 6.683405097

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM1240943_2857_8676_35213_GFP2_HG-U133A+2.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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