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Sample GSM1240934 Query DataSets for GSM1240934
Status Public on Dec 31, 2016
Title Human Mammary Epithelial Cells_Rheb_transfected_3
Sample type RNA
Source name HMECs_Rheb_transfected
Organism Homo sapiens
Characteristics cell type: Human Mammary Epithelial cells (HMECs)
genotype/variation: over-expressing Rheb oncogene
Treatment protocol Cells were brought to quisecence by serum starvation and treated with adenovirus to over-express the Rheb oncogene (10 replicates) or GFP (10 replicates). Eighteen hours after infection, activation of oncogenes and secondary targets were confirmed by western blotting.
Growth protocol Mammary epithelial cells were isolated from a breast reductive surgery at Duke University and grown at 37 degrees C in mammary epithelial cell growth medium (MEGM) containing a MEGM Bullet Kit, human transferrin and isopenterol.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a Qiagen Rneasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Gene Array Scanner (Affymetrix) following the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
Description 35204_Rheb3
Data processing The data were analyzed using the R statistical platform with the Bioconductor affymetrix package [R version 2.13.1 (2011-07-08) and Bioconductor version 2.9].
Submission date Sep 27, 2013
Last update date Dec 31, 2016
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platform ID GPL571
Series (2)
GSE51242 A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC]
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy

Data table header descriptions
VALUE Log2 GC-RMA signal.

Data table
1007_s_at 11.66747483
1053_at 6.61970464
117_at 5.556260483
121_at 7.330247186
1255_g_at 3.487740705
1294_at 7.14068216
1316_at 6.401266349
1320_at 5.59522325
1405_i_at 4.896298855
1431_at 4.138268039
1438_at 7.831828819
1487_at 8.295533957
1494_f_at 4.903244955
1598_g_at 11.12044317
160020_at 11.07097673
1729_at 8.644773419
177_at 6.127591071
1773_at 6.46814933
179_at 8.223027218
1861_at 7.243311543

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM1240934_2857_8676_35204_Rheb3_HG-U133A+2.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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