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Sample GSM1240933 Query DataSets for GSM1240933
Status Public on Dec 31, 2016
Title Human Mammary Epithelial Cells_Rheb_transfected_2
Sample type RNA
 
Source name HMECs_Rheb_transfected
Organism Homo sapiens
Characteristics cell type: Human Mammary Epithelial cells (HMECs)
genotype/variation: over-expressing Rheb oncogene
Treatment protocol Cells were brought to quisecence by serum starvation and treated with adenovirus to over-express the Rheb oncogene (10 replicates) or GFP (10 replicates). Eighteen hours after infection, activation of oncogenes and secondary targets were confirmed by western blotting.
Growth protocol Mammary epithelial cells were isolated from a breast reductive surgery at Duke University and grown at 37 degrees C in mammary epithelial cell growth medium (MEGM) containing a MEGM Bullet Kit, human transferrin and isopenterol.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a Qiagen Rneasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3-4 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The resulting biotin-labeled RNA samples were then hybridized with Affymetrix U133A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using the Gene Array Scanner (Affymetrix) following the manufacturer’s instructions (Affymetrix, Santa Clara, CA).
Description 35203_Rheb2
Data processing The data were analyzed using the R statistical platform with the Bioconductor affymetrix package [R version 2.13.1 (2011-07-08) and Bioconductor version 2.9].
 
Submission date Sep 27, 2013
Last update date Dec 31, 2016
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (2)
GSE51242 A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC]
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy

Data table header descriptions
ID_REF
VALUE Log2 GC-RMA signal.

Data table
ID_REF VALUE
1007_s_at 11.81478255
1053_at 6.363667309
117_at 5.79404639
121_at 7.410356013
1255_g_at 3.614210397
1294_at 7.396151759
1316_at 6.399894207
1320_at 5.542749571
1405_i_at 5.205455713
1431_at 3.994989276
1438_at 7.960407507
1487_at 8.19930233
1494_f_at 5.116635161
1598_g_at 11.29804889
160020_at 11.17019169
1729_at 8.599194778
177_at 6.169865456
1773_at 6.29089385
179_at 8.502580636
1861_at 6.890232852

Total number of rows: 22277

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM1240933_2857_8676_35203_Rheb2_HG-U133A+2.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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