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Sample GSM1238978 Query DataSets for GSM1238978
Status Public on Dec 31, 2015
Title NOA POST 4 (exon)
Sample type RNA
Source name testicular biopsy with nonobstructive azoospermia
Organism Homo sapiens
Characteristics Sex: male
tissue: testis
testis: right
age: 31
histopatological desription: postmeiotic arrest
response to the hormonal therapy: positive response (sperm cells appered in ejaculate
Treatment protocol Not applicable, samples extracted from untreated patients with various stages of azoospermia
Growth protocol Not applicable, total RNA extracted from testicular biopsy cells
Extracted molecule total RNA
Extraction protocol Tri-Reagent (Sigma-Aldrich, St. Louis, USA) and post-purification using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany)
Testicular biopsy
Label biotin
Label protocol The sense-strand cDNA was fragmentized and labeled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA).
Hybridization protocol Array hybridization, washing, and scanning were performed according to manufacturer’s recommendations by using GeneChip® Hybridization, Wash and Stain Kit (Affymetrix, Santa Clara, CA).
Scan protocol Microarrays scanned using Affymetrix 3000 Scanner according to the instructions of the manufacturer
Description Biomarkers project
Data processing Raw data were normalized with RMA method using Affymetrix software delivered to the scanner.
Submission date Sep 23, 2013
Last update date Dec 31, 2015
Contact name Tomasz Stokowy
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
Platform ID GPL10739
Series (1)
GSE51111 Potential biomarkers tah could help to determine the prognosis of azoospermia treatment
Reanalysis of GSM1118248

Data table header descriptions
VALUE RMA normalized

Data table
7896737 5.899456
7896739 2.657715
7896741 2.805873
7896743 9.335781
7896745 7.569304
7896747 8.917858
7896749 5.548015
7896751 3.129428
7896753 8.717559
7896755 6.762116
7896757 3.708166
7896758 4.491638
7896760 5.570146
7896762 9.023236
7896763 4.429644
7896764 5.486694
7896765 2.856116
7896766 5.701626
7896767 3.517979
7896768 8.631819

Total number of rows: 257430

Table truncated, full table size 4242 Kbytes.

Supplementary file Size Download File type/resource
GSM1238978_8P_HuGene-1_0-st-v1.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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