NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1232921 Query DataSets for GSM1232921
Status Public on Jun 30, 2014
Title PBMC_SNG001_visit4_rep24
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, SNG001
Organism Homo sapiens
Characteristics agent: SNG001
timepoint: Day 7
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 24SNGv4
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.7032719
DarkCorner 0.17685947
A_23_P117082 0.8611364
A_33_P3246448 -4.0312357
A_33_P3318220 0.1072249
A_33_P3236322 -0.18232012
A_33_P3319925 0.11904338
A_21_P0000509 -4.7437744
A_21_P0000744 -1.1561728
A_24_P215804 0.4135289
A_23_P110167 -1.1727294
A_33_P3211513 1.255064
A_23_P103349 -0.14916764
A_32_P61480 0.14433274
A_33_P3788124 0.14650202
A_33_P3414202 2.4624882
A_33_P3316686 2.358429
A_33_P3300975 -2.2027216
A_33_P3263061 0.17294307
A_33_P3261373 0.49653137

Total number of rows: 50739

Table truncated, full table size 1213 Kbytes.




Supplementary file Size Download File type/resource
GSM1232921_24SNGv4.txt.gz 21.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap