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Sample GSM1232916 Query DataSets for GSM1232916
Status Public on Jun 30, 2014
Title PBMC_SNG001_visit3_rep22
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, SNG001
Organism Homo sapiens
Characteristics agent: SNG001
timepoint: Day 4
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 22SNGv3
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.8090668
DarkCorner 0.37777424
A_23_P117082 0.010358729
A_33_P3246448 -3.9611316
A_33_P3318220 0.115493774
A_33_P3236322 -0.34984216
A_33_P3319925 0.13254194
A_21_P0000509 -4.753933
A_21_P0000744 -1.3944006
A_24_P215804 0.40623626
A_23_P110167 -0.62616736
A_33_P3211513 1.0590209
A_23_P103349 -0.08084252
A_32_P61480 0.14860195
A_33_P3788124 0.17515327
A_33_P3414202 2.8014116
A_33_P3316686 2.3743253
A_33_P3300975 -0.71525097
A_33_P3263061 -0.15934375
A_33_P3261373 0.1272431

Total number of rows: 50739

Table truncated, full table size 1215 Kbytes.




Supplementary file Size Download File type/resource
GSM1232916_22SNGv3.txt.gz 21.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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