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Sample GSM1232910 Query DataSets for GSM1232910
Status Public on Jun 30, 2014
Title PBMC_SNG001_visit3_rep20
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, SNG001
Organism Homo sapiens
Characteristics agent: SNG001
timepoint: Day 4
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 20SNGv3
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.2499201
DarkCorner 0.25633553
A_23_P117082 0.08099753
A_33_P3246448 -4.9970627
A_33_P3318220 0.20240784
A_33_P3236322 1.7580717
A_33_P3319925 1.1829244
A_21_P0000509 -5.5412083
A_21_P0000744 -1.4040345
A_24_P215804 0.6306352
A_23_P110167 -0.92196745
A_33_P3211513 1.1984243
A_23_P103349 -0.33193728
A_32_P61480 -0.21458714
A_33_P3788124 0.8084217
A_33_P3414202 1.6471453
A_33_P3316686 2.3701057
A_33_P3300975 -0.71720594
A_33_P3263061 -0.14563845
A_33_P3261373 1.0032457

Total number of rows: 50739

Table truncated, full table size 1218 Kbytes.




Supplementary file Size Download File type/resource
GSM1232910_20SNGv3.txt.gz 21.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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