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Sample GSM1232871 Query DataSets for GSM1232871
Status Public on Jun 30, 2014
Title PBMC_SNG001_visit2_rep2
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, SNG001
Organism Homo sapiens
Characteristics agent: SNG001
timepoint: Day 1
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 2SNGv2
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.1989787
DarkCorner 0.049674086
A_23_P117082 0.39674863
A_33_P3246448 -4.3045454
A_33_P3318220 0.29827014
A_33_P3236322 -0.68303007
A_33_P3319925 0.29293776
A_21_P0000509 -5.30725
A_21_P0000744 -1.5921115
A_24_P215804 0.99115425
A_23_P110167 -0.9353362
A_33_P3211513 1.2928114
A_23_P103349 -0.18276785
A_32_P61480 0.27129886
A_33_P3788124 0.2678614
A_33_P3414202 2.1060038
A_33_P3316686 1.5866442
A_33_P3300975 -0.11184985
A_33_P3263061 -0.046608925
A_33_P3261373 0.24949485

Total number of rows: 50739

Table truncated, full table size 1216 Kbytes.




Supplementary file Size Download File type/resource
GSM1232871_2SNGv2.txt.gz 21.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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