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Sample GSM1232827 Query DataSets for GSM1232827
Status Public on Jun 30, 2014
Title PBMC_placebo_visit2_rep16
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, placebo
Organism Homo sapiens
Characteristics agent: placebo
timepoint: Day 1
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 16PLCv2
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.4688435
DarkCorner 0.13553818
A_23_P117082 0.6929197
A_33_P3246448 -4.137193
A_33_P3318220 0.20452592
A_33_P3236322 0.20554212
A_33_P3319925 0.20602705
A_21_P0000509 -5.058213
A_21_P0000744 -1.2276307
A_24_P215804 2.2158566
A_23_P110167 -0.51222986
A_33_P3211513 1.2500658
A_23_P103349 0.012908367
A_32_P61480 0.20276533
A_33_P3788124 0.20141323
A_33_P3414202 2.3672094
A_33_P3316686 2.6738234
A_33_P3300975 -0.5921707
A_33_P3263061 0.0978889
A_33_P3261373 0.19170807

Total number of rows: 50739

Table truncated, full table size 1213 Kbytes.




Supplementary file Size Download File type/resource
GSM1232827_16PLCv2.txt.gz 21.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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