NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1232818 Query DataSets for GSM1232818
Status Public on Jun 30, 2014
Title PBMC_placebo_visit4_rep11
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, placebo
Organism Homo sapiens
Characteristics agent: placebo
timepoint: Day 7
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 11PLCv4
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.2833195
DarkCorner 0.2666107
A_23_P117082 0.4368067
A_33_P3246448 -3.693187
A_33_P3318220 0.34616283
A_33_P3236322 1.7678791
A_33_P3319925 0.34205243
A_21_P0000509 -5.584648
A_21_P0000744 -1.7069005
A_24_P215804 1.1403265
A_23_P110167 -0.65241337
A_33_P3211513 0.19028285
A_23_P103349 0.03336435
A_32_P61480 1.1864752
A_33_P3788124 0.32231852
A_33_P3414202 1.2177973
A_33_P3316686 1.7879268
A_33_P3300975 -0.5396652
A_33_P3263061 0.22801393
A_33_P3261373 2.2780733

Total number of rows: 50739

Table truncated, full table size 1213 Kbytes.




Supplementary file Size Download File type/resource
GSM1232818_11PLCv4.txt.gz 21.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap