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Sample GSM1232809 Query DataSets for GSM1232809
Status Public on Jun 30, 2014
Title PBMC_placebo_visit3_rep8
Sample type RNA
 
Channel 1
Source name Peripheral blood mononuclear cells, placebo
Organism Homo sapiens
Characteristics agent: placebo
timepoint: Day 4
cell type: Peripheral blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy3
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics reference: Stratagene Universal Human Reference RNA
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
Label Cy5
Label protocol Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
 
 
Hybridization protocol The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
Scan protocol Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
Description 8PLCv3
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Sep 17, 2013
Last update date Jun 30, 2014
Contact name Phillip Monk
Organization name Synairgen Research Ltd
Department Southampton General Hospital
Street address Tremona Road
City Southampton
ZIP/Postal code SO16 6YD
Country United Kingdom
 
Platform ID GPL17077
Series (1)
GSE50943 The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy3/Cy5) representing test/reference

Data table
ID_REF VALUE
GE_BrightCorner 2.5219307
DarkCorner 0.29377696
A_23_P117082 -0.15404409
A_33_P3246448 -4.1057515
A_33_P3318220 0.26851702
A_33_P3236322 0.2678408
A_33_P3319925 1.0350944
A_21_P0000509 -5.368572
A_21_P0000744 -1.1706038
A_24_P215804 1.5322433
A_23_P110167 -0.6176129
A_33_P3211513 0.762412
A_23_P103349 -0.014972759
A_32_P61480 0.25642726
A_33_P3788124 0.2542148
A_33_P3414202 1.172698
A_33_P3316686 2.4275131
A_33_P3300975 -0.6904726
A_33_P3263061 0.30045033
A_33_P3261373 0.24089962

Total number of rows: 50739

Table truncated, full table size 1214 Kbytes.




Supplementary file Size Download File type/resource
GSM1232809_8PLCv3.txt.gz 21.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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