|
Status |
Public on Jun 30, 2014 |
Title |
PBMC_placebo_visit2_rep8 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Peripheral blood mononuclear cells, placebo
|
Organism |
Homo sapiens |
Characteristics |
agent: placebo timepoint: Day 1 cell type: Peripheral blood mononuclear cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
|
|
|
Channel 2 |
Source name |
Stratagene Universal Human Reference RNA
|
Organism |
Homo sapiens |
Characteristics |
reference: Stratagene Universal Human Reference RNA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Qiagen PAXgene RNA extraction kit following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
Followed the protocol described in Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010) using 200ng of total RNA
|
|
|
|
Hybridization protocol |
The 8 pack format mastermixes were followed (Two color microarray-based gene expression analysis: Low input quick amp labeling (v 6.5, May 2010))
|
Scan protocol |
Scanned on an Agilent Technologies Scanner G2505C US84300231 using protocol GE2_107_Sep09.
|
Description |
8PLCv2
|
Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Sep 17, 2013 |
Last update date |
Jun 30, 2014 |
Contact name |
Phillip Monk |
Organization name |
Synairgen Research Ltd
|
Department |
Southampton General Hospital
|
Street address |
Tremona Road
|
City |
Southampton |
ZIP/Postal code |
SO16 6YD |
Country |
United Kingdom |
|
|
Platform ID |
GPL17077 |
Series (1) |
GSE50943 |
The effect of inhaled IFNbeta (SNG001) versus placebo on peripheral blood mononuclear cells during an upper respiratory tract infection |
|