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Sample GSM123277 Query DataSets for GSM123277
Status Public on Jul 27, 2007
Title brain_control_sampleid_mr523
Sample type RNA
 
Source name human post-mortem brain tissue, healthy control
Organism Homo sapiens
Characteristics Disease_status: Healthy control
Age (years): 52
Gender: Male
Post mortem interval (hours): 28
Braak stage: Unknown
Cause of death: Cardiac
Biomaterial provider Dr E Fuller Torrey, Stanley Medical Research Institute, 8401 Connecticut Avenue, Suite 200, Chevy Chase, Maryland, 20815, USA
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from human postmortem brain tissue using Trizol (Invitrogen, USA) or Tri reagent (Sigma, UK) total RNA isolation kits according to the manufacturer's instructions.
Label Biotin
Label protocol Double-stranded cDNA was synthesised from 5 micrograms of total RNA using Superscript II double-stranded cDNA synthesis kit (Invitrogen, USA), purified, then in-vitro transcribed and labelled using BioArray HighYield RNA transcript Labeling Kit (Enzo Life Sciences, USA)
 
Hybridization protocol Following fragmentation, 15 micrograms of cRNA were hybridised to Affymetrix Human Genome 133A GeneChips for 16 hours at 45oC with constant rotation at 60rpm. Chips were washed and stained according to manufacturer's instructions using the Affymetrix GeneChip Fluidics Station 400.
Scan protocol Affymetrix HG-U133A GeneChips were scanned with the Agilent GeneArray Scanner
Description none
Data processing The raw data (CEL files) were read into R statistical package and normalised using the robust multi-chip average (RMA) method (Irizarray et al, 'Exploration, normalization and summaries of high density oligonucleotide array probe level data', Biostatistics, 2003).
 
Submission date Jul 25, 2006
Last update date Apr 27, 2020
Contact name Sabine Bahn
E-mail(s) sb209@cam.ac.uk
Organization name University of Cambridge
Lab Cambridge Centre for Neuropsychiatric Research
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QT
Country United Kingdom
 
Platform ID GPL96
Series (1)
GSE5390 Expression profiling of human adult postmortem brain tissue from Down syndrome and healthy control subjects
Relations
Reanalyzed by GSE149466

Data table header descriptions
ID_REF Probe-set ID
VALUE RMA normalised signal intensity

Data table
ID_REF VALUE
1007_s_at 9.551309738
1053_at 5.158464509
117_at 5.945291007
121_at 7.901624217
1255_g_at 4.069192758
1294_at 6.257439648
1316_at 6.186442537
1320_at 4.318315394
1405_i_at 4.170235424
1431_at 4.202617121
1438_at 6.393158484
1487_at 7.227678223
1494_f_at 6.477370391
1598_g_at 9.362896955
160020_at 7.76946795
1729_at 7.1718338
177_at 4.567326712
1773_at 5.197044447
179_at 8.747603658
1861_at 6.292308083

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM123277.cel.gz 3.4 Mb (ftp)(http) CEL

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