NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM123272 Query DataSets for GSM123272
Status Public on Jul 27, 2007
Title brain_healthy_control_sampleid_jg1745
Sample type RNA
 
Source name human post-mortem brain tissue, healthy control
Organism Homo sapiens
Characteristics Disease_status: Healthy control
Age (years): 61
Gender: Female
Post mortem interval (hours): 30
Braak stage: 0
Cause of death: Lung cancer
Biomaterial provider Prof. Anthony Holland, Cambridge Brain Bank, Department of Pathology, Addenbrooke's Hospital, University of Cambridge, UK
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from human postmortem brain tissue using Trizol (Invitrogen, USA) or Tri reagent (Sigma, UK) total RNA isolation kits according to the manufacturer's instructions.
Label Biotin
Label protocol Double-stranded cDNA was synthesised from 5 micrograms of total RNA using Superscript II double-stranded cDNA synthesis kit (Invitrogen, USA), purified, then in-vitro transcribed and labelled using BioArray HighYield RNA transcript Labeling Kit (Enzo Life Sciences, USA)
 
Hybridization protocol Following fragmentation, 15 micrograms of cRNA were hybridised to Affymetrix Human Genome 133A GeneChips for 16 hours at 45oC with constant rotation at 60rpm. Chips were washed and stained according to manufacturer's instructions using the Affymetrix GeneChip Fluidics Station 400.
Scan protocol Affymetrix HG-U133A GeneChips were scanned with the Agilent GeneArray Scanner
Description none
Data processing The raw data (CEL files) were read into R statistical package and normalised using the robust multi-chip average (RMA) method (Irizarray et al, 'Exploration, normalization and summaries of high density oligonucleotide array probe level data', Biostatistics, 2003).
 
Submission date Jul 25, 2006
Last update date Apr 27, 2020
Contact name Sabine Bahn
E-mail(s) sb209@cam.ac.uk
Organization name University of Cambridge
Lab Cambridge Centre for Neuropsychiatric Research
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QT
Country United Kingdom
 
Platform ID GPL96
Series (1)
GSE5390 Expression profiling of human adult postmortem brain tissue from Down syndrome and healthy control subjects
Relations
Reanalyzed by GSE149466

Data table header descriptions
ID_REF Probe-set ID
VALUE RMA normalised signal intensity

Data table
ID_REF VALUE
1007_s_at 9.171363662
1053_at 5.068557623
117_at 6.07823314
121_at 7.707291297
1255_g_at 4.198792034
1294_at 6.381715392
1316_at 6.170911824
1320_at 4.526285376
1405_i_at 4.295955053
1431_at 4.152651238
1438_at 6.574458343
1487_at 7.346944665
1494_f_at 6.617158307
1598_g_at 9.420587728
160020_at 7.591121315
1729_at 7.324417304
177_at 4.412629087
1773_at 5.006300695
179_at 8.84127154
1861_at 6.253651644

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM123272.cel.gz 3.3 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap