|
| Status |
Public on Nov 01, 2014 |
| Title |
521T tumor unmatched |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
thyroid carcinoma labeled with Cyanine-3 (green)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: papillary thyroid carcinoma
gender: male
age: 37.8
histological variant: classic
multifocality: multifocal
primary tumor dimension (cm): 3.0
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 61 PTC and 13 adjacent normal thyroid tissue from total thyroidectomy was isolated by the standard Rneasy or Trizol-based method.
|
| Label |
Cy3
|
| Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| Channel 2 |
| Source name |
pool of 9 adjacent normal thyroid tissue labeled with Cyanine-5 (red)
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pool of 9 adjacent normal thyroid tissues
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 61 PTC and 13 adjacent normal thyroid tissue from total thyroidectomy was isolated by the standard Rneasy or Trizol-based method.
|
| Label |
Cy5
|
| Label protocol |
Tumor and reference samples (300ng) were differentially labeled using Low Input Quick Amp Labeling (Agilent) (Agilent Technologies, Santa Clara, CA, USA).
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Images were capture in Microarray Scanner System (Agilent), with Scan Control Software 8.1 (Agilent).
Images were quantified using Agilent Feature Extraction Software (version v.10.1.1.1).
|
| Description |
target sample Cy3-labeled mixed with reference sample Cy5-labeled
|
| Data processing |
Numeric data was generated and normalized by intensity-dependent global normalization (LOWESS) with Feature Extraction v10.1 software (Agilent)
|
| |
|
| Submission date |
Sep 16, 2013 |
| Last update date |
Nov 01, 2014 |
| Contact name |
mateus camargo barros filho |
| E-mail(s) |
mateuscbarros@gmail.com
|
| Organization name |
AC Camargo Cancer Center
|
| Street address |
Tagua Street 440
|
| City |
Sao Paulo |
| State/province |
SP |
| ZIP/Postal code |
05409011 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE50901 |
Expression profiling identifies deregulated pathways and top diagnostic markers in papillary thyroid carcinoma |
|
