|
| Status |
Public on Dec 01, 2014 |
| Title |
HMLEN-SNAIL; replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HMLEN cells transduced with pLEX-SNAIL and pLEX-MCS lentiviruses.
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMLEN
treatment: SNAIL
emt phenotype: Mesenchymal
colony formation ability: Strong
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy5
|
| Label protocol |
The testing RNA samples and universal human reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Universal Mouse Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Universal Mouse Reference RNA
cell line: Mix of 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy3
|
| Label protocol |
The testing RNA samples and universal human reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Each cy5 labeled testing sample was mixed with equal amount cy3-labeled reference sample and fragmented and blocked (Agilent Gene Expression Hybridization Kit), and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
Images were quantified using Agilent Feature Extraction Software (version 9.5.3.1).
|
| Description |
See Agilent Catalog #740000 for detail
Stable cell lines were generated using pLEX based lentivirus system and selected for puromycin resistance. Cells were then cultured without puromycin in normal mammaliam cell culture condition till they were about 90% conflunecy before harvesting.
|
| Data processing |
Agilent Feature Extraction Software (v 9.5.3.1) was used for background subtraction and linear Lowess normalization.
|
| |
|
| Submission date |
Sep 16, 2013 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Yong Wei |
| E-mail(s) |
yongwei@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kang
|
| Street address |
LTL227 Wathington Road
|
| City |
Plainsboro |
| State/province |
NJ |
| ZIP/Postal code |
08536 |
| Country |
USA |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE50888 |
HMLEN cells with Vector Control vs SNAIL vs SNAIL/FBXO11 |
| GSE50889 |
Vector Control vs SNAIL vs SNAIL/FBXO11 |
|
