|
| Status |
Public on Sep 14, 2013 |
| Title |
genomic DNA from microdissected human kidney79 |
| Sample type |
genomic |
| |
|
| Source name |
kidney 79
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney tubule
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
1mm x 1mm biopsy tissues were placed under an Olympus model SZX12 stereomicroscope using 90x magnification. The biopsy tissue was manually microdissected at 4°C in RNALater for glomerular and tubular compartments using fine tip forceps. The corresponding tubulointerstitial and vascular compartment was placed into RLT solution. For easier designation we called this component tubules. Dissected tissue was homogenized using a Powergen125 (Fisher) homogenizer and stored at -80°C. Genomic DNA was prepared using DNA extraction kit (Qiagen) according to manufacturer's instructions.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
|
| Data processing |
GenomeStudio V2011.1; methylation module 1.9.0
|
| |
|
| Submission date |
Sep 13, 2013 |
| Last update date |
Sep 14, 2013 |
| Contact name |
Yi-An Ko |
| E-mail(s) |
yian.kia.ko@gmail.com
|
| Organization name |
UPeenn
|
| Department |
Genetics & Gene Regulation
|
| Lab |
Susztak
|
| Street address |
415 curie blvd
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE50874 |
Methylation Profiling of Human Kidney Tubules |
|
