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Sample GSM1229968 Query DataSets for GSM1229968
Status Public on Sep 13, 2013
Title input DNA
Sample type SRA
Source name Innate type-2 lymphocytes
Organism Mus musculus
Characteristics strain: C57BL/6
cell type/growth condition: ILC2s were sorted from the MLN of IL-25-treated mice
antibody: none
Extracted molecule genomic DNA
Extraction protocol ~3 x 10^7 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments.
Standard Illumina kit was used for library construction.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
Data processing Base calling was performed using Illumina software pipeline (Genome Analyzer)
Align to the mouse genome (mm10) using bowtie (version 0.12.8) allowing 1 mismatch and reporting only uniquely aligning hits.
Peak calling was perfomed using (QuEST) v2.4 with input chromatin as control.
Genome_build: mm10
Supplementary_files_format_and_content: Peaks listed with genomic coordinates and QuEST score
Submission date Sep 12, 2013
Last update date May 15, 2019
Contact name Aly Azeem Khan
Organization name University of Chicago
Street address 6045 S. Kenwood Ave.
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platform ID GPL11002
Series (1)
GSE50806 Specification of innate type-2 lymphocytes by the transcriptional determinant Gfi1
BioSample SAMN02355913
SRA SRX349377

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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