|
Status |
Public on Sep 13, 2013 |
Title |
SUBJ2_GLIA_EXPER2 |
Sample type |
genomic |
|
|
Source name |
genomic DNA from nonneuronal cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: nonneuronal cells gender: Male age: adult tissue: orbitofrontal cortex disease state: normal replicate experiment: 2
|
Extracted molecule |
genomic DNA |
Extraction protocol |
neuronal and nonneuronal nuclei from human postmortem brain material were isolated by fluorescence-activated sorting, as described in Kozlenkov et.al., (2013), Nucleic Acids Research (accepted for publication on 28-Aug 2013) genomic DNA was isolated by proteinase K treatment, phenol extraction and ethanol precipitation
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
FACS-isolated nuclei from postmortem human brain
|
Data processing |
GenomeStudio 2011.1 (Illumina), Methylation v1.9 module
|
|
|
Submission date |
Sep 11, 2013 |
Last update date |
Sep 13, 2013 |
Contact name |
Stella Dracheva |
E-mail(s) |
stella.dracheva@mssm.edu
|
Organization name |
Icahn School of Medicine at Mount Sinai
|
Department |
Dept. of Psychiatry
|
Street address |
James J. Peters VA Medical Center,130 W Kingsbridge Rd
|
City |
Bronx |
State/province |
New York |
ZIP/Postal code |
10468 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE50798 |
Differences in DNA methylation between human neuronal and glial cells are concentrated in enhancers and non-CpG sites [methylation array] |
GSE50853 |
Differences in DNA methylation between human neuronal and glial cells are concentrated in enhancers and non-CpG sites |
|