|
| Status |
Public on Sep 13, 2013 |
| Title |
SUBJ6_GLIA_EXPER1 |
| Sample type |
genomic |
| |
|
| Source name |
genomic DNA from nonneuronal cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: nonneuronal cells
gender: Male
age: adult
tissue: orbitofrontal cortex
disease state: normal
replicate experiment: 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
neuronal and nonneuronal nuclei from human postmortem brain material were isolated by fluorescence-activated sorting, as described in Kozlenkov et.al., (2013), Nucleic Acids Research (accepted for publication on 28-Aug 2013)
genomic DNA was isolated by proteinase K treatment, phenol extraction and ethanol precipitation
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
FACS-isolated nuclei from postmortem human brain
|
| Data processing |
GenomeStudio 2011.1 (Illumina), Methylation v1.9 module
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
Sep 13, 2013 |
| Contact name |
Stella Dracheva |
| E-mail(s) |
stella.dracheva@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Dept. of Psychiatry
|
| Street address |
James J. Peters VA Medical Center,130 W Kingsbridge Rd
|
| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10468 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE50798 |
Differences in DNA methylation between human neuronal and glial cells are concentrated in enhancers and non-CpG sites [methylation array] |
| GSE50853 |
Differences in DNA methylation between human neuronal and glial cells are concentrated in enhancers and non-CpG sites |
|
