|
| Status |
Public on Mar 27, 2014 |
| Title |
PMN cells donor 2 |
| Sample type |
genomic |
| |
|
| Source name |
bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Polymorphonuclear neutrophil
gender: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted with Trizol.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450K Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using Iscan Array scanner using standard recommended Illumina scanner setting
|
| Description |
cells isolated from healthy bone marrow
|
| Data processing |
GenomeStudio software v2011.1
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
Mar 27, 2014 |
| Contact name |
Karl Ekwall |
| E-mail(s) |
karl.ekwall@ki.se
|
| Phone |
+46 8 6089133
|
| Organization name |
Karolinska Inst
|
| Street address |
Alfred Nobels Alle 7
|
| City |
Stockholm |
| ZIP/Postal code |
S-141 89 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE50797 |
Analysis of the DNA methylome and transcriptome in granulopoiesis reveal timed changes and dynamic enhancer methylation |
|
