|
| Status |
Public on Jul 31, 2015 |
| Title |
LPS-DC |
| Sample type |
RNA |
| |
|
| Source name |
BMDCs stimulated with LPS
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: bone marrow dendritic cell
treatment: LPS
|
| Treatment protocol |
DCs were incubated with either PBS, LPS (1 µg/mL) or PTX (100 ng/mL) for 24 h.
|
| Growth protocol |
Femal C57BL6 is grow in SPF condition until 6 weeks. Dendritic cells were generated from bone marrow cells.Cells were cultured in medium supplemented with 20 ng/ml of murine rGM-CSF. Nonadherent cells were removed carefully, and fresh medium was added every 2 days. On day 7, nonadherent cells that were released spontaneously from proliferating cell clusters were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
start with 3μg total RNA, and Biotinylated cRNA were prepared according to the standard Affymetrix protocol, in which, One-Cycle Eukaryotic Target Labeling Assay(Lot no. 900493) is used in the experiment.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse 430_2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.Eukaryotic Target Hybridization,Lot no.900720, is used for the experiment.
|
| Scan protocol |
the chips were scanned withthe affymetrix genechip 7G plus, and GCOS v1.0 is used for the image processing.
|
| Description |
gene expression data from BMDCs stimulated with LPS
|
| Data processing |
genespring 10.0, is used for automatic normalizing the chip data and analysis the fold change.
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
Jul 31, 2015 |
| Contact name |
haiyan zhou |
| E-mail(s) |
hyzhou02@sibcb.ac.cn
|
| Organization name |
Shanghai Institute of Biochemistry and Cell Biology
|
| Street address |
No.320Yueyang Road
|
| City |
shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE50779 |
Expression data from DCs stimulated with LPS vs DCs stimulated with PTX |
|
